| Applications: | Immunodepletion/Immunocompetition |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Phospho-PRP19 Blocking Peptide is synthetically produced from the 200-300 sequence and is suitable for use in western blot applications. |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Prpf19 |
| Gene ID: | 246216 |
| Uniprot ID: | PRP19_RAT |
| Immunogen Region: | 200-300 |
| Immunogen: | Phosphorylated synthetic peptide taken within amino acid region 200-300 on rat PRP19F protein. |
| Function | Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome. |
| Peptide Name | Pre-Mrna-Processing Factor 19 Neuronal Differentiation-Related Gene Protein Prp19/Pso4 Homolog Ring-Type E3 Ubiquitin Transferase Prp19 |
| Database Links | Reactome: R-RNO-6781823 Reactome: -RNO-6782135 Reactome: -RNO-6782210 Reactome: -RNO-72163 |
| Cellular Localisation | Nucleus Nucleoplasm Cytoplasm Cytoskeleton Spindle Lipid Droplet Nucleoplasmic In Interphase Cells Irregularly Distributed In Anaphase Cells In Prophase Cells Uniformly Distributed But Not Associated With Condensing Chromosomes Found In Extrachromosomal Regions In Metaphase Cells Mainly Localized To The Mitotic Spindle Apparatus When Chromosomes Segregate During Anaphase When Nuclei Reform During Late Telophase Uniformly Distributed In Daughter Cells And Displays No Preferred Association With Decondensing Chromatin Recruited On Damaged Dna At Sites Of Double-Strand Break |
| Alternative Peptide Names | Pre-Mrna-Processing Factor 19 protein Neuronal Differentiation-Related Gene Protein protein Prp19/Pso4 Homolog protein Ring-Type E3 Ubiquitin Transferase Prp19 protein Prpf19 protein Prp19 protein |
Information sourced from Uniprot.org