Primary antibodies

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Primary antibodies

Primary antibodies are immunoglobulins which are raised against an antigen with a specific amino acid sequence, and can be used in laboratory research for qualification and quantification of protein expression. Typically primary antibodies are available in monoclonal or polyclonal forms, depending on the way they are synthesised. Primary antibodies are often raised in animals, such as mice or rabbits, and are then purified and characterized for their specificity and affinity to the antigen of interest, with each offering their own strengths and unique properties.

 

A single primary antibody may been used in various laboratory techniques, including ELISA, Western blot, and Immunofluorescence, which offers researchers a wide range of methods to detect and quantify an antigen of interest in a sample. Primary antibodies are typically used in combination with secondary antibodies, which are labeled with a fluorescent or enzymatic marker, to create a signal that can be easily visualized or measured

 

We have a wide range of primary antibodies which have been successfully used in assays like ELISA, western blot, immunohistochemistry and flow cytometry. With over 50,000 primary antibodies on our platform we aim to support the majority of research areas.

 

Search and shop our wide selection of high-quality primary antibodies using the links below, or for more technical information please use the resources in the side menu.