Anti-MAP2K1 antibodies

Immunohistochemistry analysis of MEK1 in paraffin-embedded mouse kidney using [KO Validated] MEK1 Rabbit polyclonal antibody (STJ114560) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAP2K1 antibody (1-100) (STJ114560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of U2OS cells using MEK1 Rabbit polyclonal antibody (STJ24463). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP2K1 antibody (1-100) (STJ24463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain using 3 Mu g MEK1 antibody (STJ11101700). Western blot was performed from the immunoprecipitate using MEK1 (STJ11101700) at a dilution of 1:1000.

Anti-MAP2K1 antibody (1-100) [S0MR] (STJ11101700)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of MEK1 in (1) A431 lysates; (2) HeLa lysates using MEK1 antibody.

Anti-MAP2K1 antibody (1-40 aa) [R07-8C5] (STJA0015763)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat/Cow/Dog

£264.50

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Western blot analysis of 293T-UV cells using MEK-1 Polyclonal Antibody diluted at 1：500

Anti-MAP2K1 antibody (252-301 aa) (STJ94076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of mouse brain with Anti-MAP2K1 primary antibody (STJ501657) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-MAP2K1 antibody (300-350) (STJ501657)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAP2K1 antibody (300-350) {Biotin} (STJ501658)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAP2K1 antibody (300-350) {FITC} (STJ501659)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of MEK1 at dilution of 1:1000

Anti-MEK1 antibody [SM5090] (STJA0005090)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-MEK1 antibody [SM5178] (STJA0005178)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Western blot analysis of lysates from C6 cells, using Phospho-MEK1-pS221 Rabbit pAb (STJ22232) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K1-pS221 antibody (STJ22232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-S217 Rabbit pAb (STJ114560). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K1-S217 antibody (STJ22230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit pAb (STJ22231) at 1:1000 dilution or MEK1 antibody (STJ114560). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAP2K1-S298 antibody (STJ22231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb (STJ11102602) at 1:1000 dilution. Both HeLa cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PMA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-MAP2K1-S298 antibody [S2MR] (STJ11102602)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Western blot analysis of HEPG2-UV using p-MEK-1 (S298) antibody. Antibody was diluted at 1:500

Anti-Phospho-MAP2K1-Ser298 antibody (264-313 aa) (STJ90489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Phospho-MEK1-T286 Rabbit pAb (STJ22233) at 1:2000 dilution or MEK1 antibody (STJ114560). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by ATP (5 mM) at 30 °C for 1 hour. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by ATP (5 mM) at 30 °C for 1 hour. PC-12 cells were treated by Nocodazole (1 Mu g/mL) at 37 °C for 16 hours or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-MAP2K1-T286 antibody (STJ22233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from C6 cells, using Phospho-MEK1-T292 Rabbit mAb (STJ11102603) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-MAP2K1-T292 antibody [S3MR] (STJ11102603)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAP2K1-Thr286 antibody (252-301 aa) (STJ90854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-MEK-1 (T292) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MAP2K1-Thr292 antibody (261-310 aa) (STJ90326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of recombinant wild type MEK 1 (WT) and mutant MEK 1 (T292A) (Mut). Specific immunolabeling of the ~45 kDa MEK-1 protein phosphorylated at Thr292 is shown in the second lane where MAP kinase was coexpressed.

Anti-Phospho-MAP2K1-Thr292 antibody (STJA0003684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Bovine/Canine/Non-Human Primates/Rat

£454.50

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Western blot analysis of 3T3-EGF cells using Phospho-MEK-1 (T386) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K1-Thr386 antibody (344-393 aa) (STJ90585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of human T47D cells showing specific immunolabeling of the ~45 kDa MEK 1 protein phosphorylated at Thr386 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-MAP2K1-Thr386 antibody (STJA0003685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Bovine/Canine/Chicken/Non-Human Primates/Mouse/Xenopus

£454.50

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Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (-) or incubated in the presence of serum (+). Immunolabeling of an additional band at ~95 kDa was also observed.

Anti-Phospho-MAP2K1/2-Ser218/222 antibody (STJA0003683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Human/Bovine/Canine/Chicken/Non-Human Primates/Rat/Xenopus/Zebrafish

£454.50

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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Phospho-MEK-1/2-Ser218/222 antibody (189-238 aa) (STJ90327)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1/2 (Phospho-Ser221) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MEK-1/2-Ser222/226 antibody (193-242 aa) (STJ90328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-T292 Rabbit pAb (STJ11103753) at 1:1000 dilution. HeLa cells were treated by Nocodazole (100 ng/ml) at 37 °C for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MEK1-T292 antibody (STJ11103753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Anti-Recombinant-MEK1 antibody [SM0650] (STJA0010650)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-Recombinant-MEK1 antibody [SM0690] (STJA0010690)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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MAP2K1 antibodies

Target Information

Synonyms

Anti-MAP2K1 antibody (1-100) (STJ114560)

Anti-MAP2K1 antibody (1-100) (STJ24463)

Anti-MAP2K1 antibody (1-100) [S0MR] (STJ11101700)

Anti-MAP2K1 antibody (1-40 aa) [R07-8C5] (STJA0015763)

Anti-MAP2K1 antibody (252-301 aa) (STJ94076)

Anti-MAP2K1 antibody (300-350) (STJ501657)

Anti-MAP2K1 antibody (300-350) {Biotin} (STJ501658)

Anti-MAP2K1 antibody (300-350) {FITC} (STJ501659)

Anti-MEK1 antibody [SM5090] (STJA0005090)

Anti-MEK1 antibody [SM5178] (STJA0005178)

Anti-Phospho-MAP2K1-pS221 antibody (STJ22232)

Anti-Phospho-MAP2K1-S217 antibody (STJ22230)

Anti-Phospho-MAP2K1-S298 antibody (STJ22231)

Anti-Phospho-MAP2K1-S298 antibody [S2MR] (STJ11102602)

Anti-Phospho-MAP2K1-Ser298 antibody (264-313 aa) (STJ90489)

Anti-Phospho-MAP2K1-T286 antibody (STJ22233)

Anti-Phospho-MAP2K1-T292 antibody [S3MR] (STJ11102603)

Anti-Phospho-MAP2K1-Thr286 antibody (252-301 aa) (STJ90854)

Anti-Phospho-MAP2K1-Thr292 antibody (261-310 aa) (STJ90326)

Anti-Phospho-MAP2K1-Thr292 antibody (STJA0003684)

Anti-Phospho-MAP2K1-Thr386 antibody (344-393 aa) (STJ90585)

Anti-Phospho-MAP2K1-Thr386 antibody (STJA0003685)

Anti-Phospho-MAP2K1/2-Ser218/222 antibody (STJA0003683)

Anti-Phospho-MEK-1/2-Ser218/222 antibody (189-238 aa) (STJ90327)

Anti-Phospho-MEK-1/2-Ser222/226 antibody (193-242 aa) (STJ90328)

Anti-Phospho-MEK1-T292 antibody (STJ11103753)

Anti-Recombinant-MEK1 antibody [SM0650] (STJA0010650)

Anti-Recombinant-MEK1 antibody [SM0690] (STJA0010690)