| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
PDE4D5 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | Expressed in colonic epithelial cells (at protein level). Widespread.most abundant in skeletal muscle. Isoform 6: Detected in brain. Isoform 8: Detected in brain, placenta, lung and kidney. Isoform 7: Detected in heart and skeletal muscle. |
| Post Translational Modifications | Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation. Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| Peptide Name | Camp-Specific 3' -5'-Cyclic Phosphodiesterase 4dDpde3Pde43 |
| Database Links | Reactome: R-HSA-180024Reactome: R-HSA-418555 |
| Cellular Localisation | Apical Cell MembraneCytoplasmMembraneCytoskeletonMicrotubule Organizing CenterCentrosomeFound In The Soluble FractionAssociated With MembranesAnd Associated With The Cytoskeleton And The CentrosomeColocalized With Shank2 To The Apical Membrane Of Colonic Crypt Cells |
| Alternative Peptide Names | Camp-Specific 3' -5'-Cyclic Phosphodiesterase 4d proteinDpde3 proteinPde43 proteinPDE4D proteinDPDE3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
