| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This Tnfsf10 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Tnfsf10 |
| Gene ID: |
22035 |
| Uniprot ID: |
TNF10_MOUSE |
| Specificity: |
This kit recognizes Mouse TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Mouse TRAIL/TNFSF10 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Tyrosine phosphorylated by PKDCC/VLK. |
| Function | Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. |
| Protein Name | Tumor Necrosis Factor Ligand Superfamily Member 10Tnf-Related Apoptosis-Inducing LigandProtein TrailCd Antigen Cd253 |
| Database Links | Reactome: R-MMU-3371378Reactome: -MMU-5218900Reactome: -MMU-69416Reactome: -MMU-75158 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinSecretedExists Both As Membrane-Bound And Soluble Form |
| Alternative ELISA Names | Tumor Necrosis Factor Ligand Superfamily Member 10 ELISA kitTnf-Related Apoptosis-Inducing Ligand ELISA kitProtein Trail ELISA kitCd Antigen Cd253 ELISA kitTnfsf10 ELISA kitTrail ELISA kit |
| Specificity | This kit recognizes Mouse TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Mouse TRAIL/TNFSF10 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-105 | 97 |
| EDTA plasma (n=8) | 93-107 | 99 |
| Cell culture media (n=8) | 92-105 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 50.22 | 124.92 | 346.87 | 49.84 | 136.70 | 361.81 |
| Standard deviation | 2.69 | 5.80 | 11.34 | 2.82 | 5.78 | 13.28 |
| CV (%) | 5.36 | 4.64 | 3.27 | 5.66 | 4.23 | 3.67 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
