Mouse Tnfsf10 (Tumor necrosis factor ligand superfamily member 10) Sandwich ELISA Kit (STJE0004988)

Name: Mouse Tnfsf10 (Tumor necrosis factor ligand superfamily member 10) Sandwich ELISA Kit (STJE0004988)
SKU: STJE0004988
Availability: InStock

⇓ Datasheet ⇓ SDS Citations

SPECIFICATIONS

STJE0004988

Current Stock:

🚚 Free UK Delivery on orders over £150

Quantity:

Processing The item has been added

Enquire For Bulk Order

✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short Description	This Tnfsf10 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	Tnfsf10
Gene ID	22035
Uniprot ID	TNF10_MOUSE
Specificity	This kit recognizes Mouse TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Mouse TRAIL/TNFSF10 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Post Translational Modifications	Tyrosine phosphorylated by PKDCC/VLK.
Function	Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Protein Name	Tumor Necrosis Factor Ligand Superfamily Member 10 Tnf-Related Apoptosis-Inducing Ligand Protein Trail Cd Antigen Cd253
Database Links	Reactome: R-MMU-3371378 Reactome: -MMU-5218900 Reactome: -MMU-69416 Reactome: -MMU-75158
Cellular Localisation	Cell Membrane Single-Pass Type Ii Membrane Protein Secreted Exists Both As Membrane-Bound And Soluble Form
Alternative ELISA Names	Tumor Necrosis Factor Ligand Superfamily Member 10 ELISA kit Tnf-Related Apoptosis-Inducing Ligand ELISA kit Protein Trail ELISA kit Cd Antigen Cd253 ELISA kit Tnfsf10 ELISA kit Trail ELISA kit
Specificity	This kit recognizes Mouse TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Mouse TRAIL/TNFSF10 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-105	97
EDTA plasma (n=8)	93-107	99
Cell culture media (n=8)	92-105	99

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	50.22	124.92	346.87	49.84	136.70	361.81
Standard deviation	2.69	5.80	11.34	2.82	5.78	13.28
CV (%)	5.36	4.64	3.27	5.66	4.23	3.67

Citations

View product citations for antibody STJE0004988 on CiteAb