• Mouse Tnfsf10 (Tumor necrosis factor ligand superfamily member 10) Sandwich ELISA (STJE0004988)

Mouse Tnfsf10 (Tumor necrosis factor ligand superfamily member 10) Sandwich ELISA (STJE0004988)

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STJE0004988

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This Tnfsf10 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Tnfsf10
Gene ID: 22035
Uniprot ID: TNF10_MOUSE
Specificity: This kit recognizes Mouse TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Mouse TRAIL/TNFSF10 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Tyrosine phosphorylated by PKDCC/VLK.
Function Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Protein Name Tumor Necrosis Factor Ligand Superfamily Member 10
Tnf-Related Apoptosis-Inducing Ligand
Protein Trail
Cd Antigen Cd253
Database Links Reactome: R-MMU-3371378
Reactome: -MMU-5218900
Reactome: -MMU-69416
Reactome: -MMU-75158
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Secreted
Exists Both As Membrane-Bound And Soluble Form
Alternative ELISA Names Tumor Necrosis Factor Ligand Superfamily Member 10 ELISA kit
Tnf-Related Apoptosis-Inducing Ligand ELISA kit
Protein Trail ELISA kit
Cd Antigen Cd253 ELISA kit
Tnfsf10 ELISA kit
Trail ELISA kit
Specificity This kit recognizes Mouse TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Mouse TRAIL/TNFSF10 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-105 97
EDTA plasma (n=8) 93-107 99
Cell culture media (n=8) 92-105 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 50.22 124.92 346.87 49.84 136.70 361.81
Standard deviation 2.69 5.80 11.34 2.82 5.78 13.28
CV (%) 5.36 4.64 3.27 5.66 4.23 3.67
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance