| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <0.1 EU/Mu g |
| Immunoreactivity: | Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 25.33-101.30 ng/mL, corresponding to a specific activity of 9.87×103 ~3.95×10< |
| Gene Symbol: | Mfge8 |
| Gene ID: | 17304 |
| Uniprot ID: | MFGM_MOUSE |
| Immunogen: | Recombinant Mouse MFG-E8 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala23-Cys463) of mouse MFGE8/BA46 (Accession #NM_008594.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | ASGDFCDSSLCLNGGTCLTG QDNDIYCLCPEGFTGLVCNE TERGPCSPNPCYNDAKCLVT LDTQRGDIFTEYICQCPVGY SGIHCETETNYYNLDGEYMF TTAVPNTAVPTPAPTPDLSN NLASRCSTQLGMEGGAIADS QISASSVYMGFMGLQRWGPE LARLYRTGIVNAWTASNYDS KPWIQVNLLRKMRVSGVMTQ GASRAGRAEYLKTFKVAYSL DGRKFEFIQDESGGDKEFL |
| Post Translational Modifications | N-glycosylated. Isoform 1 also exists in both an O-glycosylated and a non-O-glycosylated form. |
| Function | Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. |
| Protein Name | LactadherinMfgmMilk Fat Globule-Egf Factor 8Mfg-E8Sed1Sperm Surface Protein Sp47Mp47 |
| Database Links | Reactome: R-MMU-381426Reactome: -MMU-8957275 |
| Cellular Localisation | MembranePeripheral Membrane ProteinSecretedCytoplasmic VesicleSecretory VesicleAcrosome MembraneLocated In The Acrosomal Region Of Zona-Pellucida Bound Sperm |
| Alternative Protein Names | Lactadherin proteinMfgm proteinMilk Fat Globule-Egf Factor 8 proteinMfg-E8 proteinSed1 proteinSperm Surface Protein Sp47 proteinMp47 proteinMfge8 protein |
Information sourced from Uniprot.org