• Biotinylated Mouse GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Mouse GFRAL is greater than 95% as determined by SEC-HPLC.
  • Immobilized Biotinylated Mouse GFRAL, His Tag at 0. 5 Mu g/ml (100 Mu l/Well) on streptavidin (5 Mu g/ml) precoated plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6. 2ng/ml determined by ELISA.

Mouse GFRAL/GFR alpha-like protein (Recombinant) (N-His-Avi) {Biotin} (STJP001652)

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STJP001652

£672.50 - £2,092.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Mouse GFRAL/GFR alpha-like-N-His-Avi protein was developed from hek293 and has a target region of N-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: Gfral
Gene ID: 404194
Uniprot ID: GFRAL_MOUSE
Immunogen Region: Gln20-Glu350
Immunogen: DNA sequence encoding Mouse GFRAL/GFR alpha-like including a N-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Cleaved and inactivated by MMP14, inhibiting the GDF15-GFRAL aversive response.
Function Brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. The GDF15-GFRAL aversive response is triggered by stresses, such as anticancer drugs (camptothecin or cisplatin), cancers or drugs such as metformin. Upon interaction with its ligand, GDF15, mediates the GDF15-induced autophosphorylation and activation of the RET tyrosine kinase receptor, leading to activation of MAPK- and AKT- signaling pathways. Ligand-binding activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues.
Protein Name Gdnf Family Receptor Alpha-Like
Cellular Localisation Cell Membrane
Single-Pass Membrane Protein
Extracellular Side
Alternative Protein Names Gdnf Family Receptor Alpha-Like protein
Gfral protein
Gral protein

Information sourced from Uniprot.org

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