• Western blot analysis of decreasing concentrations of total rabbit IgG reduced and denatured in 2X SDS loading buffer with DTT using Mouse Anti-Rabbit IgG (Heavy-Chain Specific) mAb (1:1000 diluted).
  • Immunoprecipitation analysis of CHO-K1 cell lysates transfected with HA-tagged NGAL using HA-Tag Rabbit pAb (390001, 1:100 diluted) (lane 2). Lane 1 contains rabbit IgG IP control lysate. All lanes are probed with HA-Tag Rabbit pAb (390001, 1:1000 diluted) as the primary antibody. Panel A incubated with secondary antibodies Anti-Rabbit whole IgG, HRP-linked Antibody for detecting.Panel B incubated with Anti-Rabbit IgG (Heavy-Chain Specific) mAb (1:50000 diluted) and the Anti-mouse whole IgG, HRP-linked Antibody was used for detecting.
  • Western blot detection of MEK-2 in THP-1, Jurkat, Raji, 3T3 and Hela cell lysates using 310001 (MEK-2 rabbit pAb, 1:1000 diluted) , then incubated with 201069 (Rabbit IgG mAb, 1:50000 diluted).Anti-mouse IgG, HRP-linked Antibody was used for detecting.Predicted band size:44KDa.Observed band size:44KDa.

Mouse Anti-Rabbit IgG antibody {Unconjugated} (STJ99246)

SKU:
STJ99246

$187.65 - $453.60
Current Stock:
Host: Mouse
Applications: WB/ELISA
Reactivity: Rabbit
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Unconjugated mouse monoclonal anti-Rabbit IgG secondary antibody. For use in most research applications.
Clonality: Monoclonal
Clone ID: 5B2-F9-D6
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:50000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: This antibody detects rabbit IgG.
Immunogen: Rabbit IgG
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance