| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | MAPK3 Positive Control for STJ501703 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: | WB: 1:500 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | MAPK3 |
| Gene ID: | 5595 |
| Uniprot ID: | MK03_HUMAN |
| Specificity: | This is positive control is recommended for use in combination with MAPK3 antibody STJ501703. |
| Post Translational Modifications | Phosphorylated upon KIT and FLT3 signaling. Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204. Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination.leading to activation. Deubiquitinated by CYLD. |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. |
| Peptide Name | Mitogen-Activated Protein Kinase 3Map Kinase 3Mapk 3Ert2Extracellular Signal-Regulated Kinase 1Erk-1Insulin-Stimulated Map2 KinaseMap Kinase Isoform P44P44-MapkMicrotubule-Associated Protein 2 KinaseP44-Erk1 |
| Database Links | Reactome: R-HSA-110056Reactome: R-HSA-112409Reactome: R-HSA-1169408Reactome: R-HSA-1181150Reactome: R-HSA-1295596Reactome: R-HSA-1502540Reactome: R-HSA-162658 P27361-3Reactome: R-HSA-170968Reactome: R-HSA-198753Reactome: R-HSA-202670Reactome: R-HSA-2029482Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-2559580Reactome: R-HSA-2559582Reactome: R-HSA-2559585Reactome: R-HSA-2871796Reactome: R-HSA-3371453Reactome: R-HSA-375165Reactome: R-HSA-444257Reactome: R-HSA-445144Reactome: R-HSA-450341Reactome: R-HSA-456926Reactome: R-HSA-5654726Reactome: R-HSA-5654727Reactome: R-HSA-5654732Reactome: R-HSA-5654733Reactome: R-HSA-5663213Reactome: R-HSA-5668599Reactome: R-HSA-5673001Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-5675221Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-6811558Reactome: R-HSA-73728Reactome: R-HSA-74749Reactome: R-HSA-877300Reactome: R-HSA-879415Reactome: R-HSA-881907Reactome: R-HSA-8939211Reactome: R-HSA-8940973Reactome: R-HSA-8943724Reactome: R-HSA-9627069Reactome: R-HSA-9634638Reactome: R-HSA-9635465Reactome: R-HSA-9649948Reactome: R-HSA-9652169Reactome: R-HSA-9656223Reactome: R-HSA-9664422Reactome: R-HSA-9725371Reactome: R-HSA-9732724Reactome: R-HSA-9768919Reactome: R-HSA-982772 |
| Cellular Localisation | CytoplasmNucleusMembraneCaveolaCell JunctionFocal AdhesionAutophosphorylation At Thr-207 Promotes Nuclear LocalizationPea15-Binding Redirects The Biological Outcome Of Mapk3 Kinase-Signaling By Sequestering Mapk3 Into The Cytoplasm |
| Alternative Peptide Names | Mitogen-Activated Protein Kinase 3 proteinMap Kinase 3 proteinMapk 3 proteinErt2 proteinExtracellular Signal-Regulated Kinase 1 proteinErk-1 proteinInsulin-Stimulated Map2 Kinase proteinMap Kinase Isoform P44 proteinP44-Mapk proteinMicrotubule-Associated Protein 2 Kinase proteinP44-Erk1 proteinMAPK3 proteinERK1 proteinPRKM3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance