| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 12-48 ng/mL in the presence of 10 ng/mL of recombinant human NGF. |
| Gene Symbol: | NTRK1 |
| Gene ID: | 4914 |
| Uniprot ID: | NTRK1_HUMAN |
| Immunogen Region: | Ala33-Glu407 |
| Immunogen: | Recombinant Human Trk-A/NTRK1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala33-Glu407) of human TrkA (Accession #NP_001012331.1) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Sequence: | AAPCPDACCPHGSSGLRCTR DGALDSLHHLPGAENLTELY IENQQHLQHLELRDLRGLGE LRNLTIVKSGLRFVAPDAFH FTPRLSRLNLSFNALESLSW KTVQGLSLQELVLSGNPLHC SCALRWLQRWEEEGLGGVPE QKLQCHGQGPLAHMPNASCG VPTLKVQVPNASVDVGDDVL LRCQVEGRGLEQAGWILTEL EQSATVMKSGGLPSLGLTLA NVTSDLNRKNVTCWAENDV |
| Post Translational Modifications | Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1. N-glycosylated. Isoform TrkA-I and isoform TrkA-II are N-glycosylated. Ubiquitinated. Undergoes polyubiquitination upon activation.regulated by NGFR. Ubiquitination by NEDD4L leads to degradation. Ubiquitination regulates the internalization of the receptor. |
| Function | Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand. Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. Isoform TrkA-III: Resistant to NGF, it constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. |
| Protein Name | High Affinity Nerve Growth Factor Receptor Neurotrophic Tyrosine Kinase Receptor Type 1 Trk1-Transforming Tyrosine Kinase Protein Tropomyosin-Related Kinase A Tyrosine Kinase Receptor Tyrosine Kinase Receptor A Trk-A Gp140trk P140-Trka |
| Database Links | Reactome: R-HSA-167021 Reactome: R-HSA-167044 Reactome: R-HSA-170968 P04629-1 Reactome: R-HSA-170984 Reactome: R-HSA-177504 Reactome: R-HSA-187024 Reactome: R-HSA-187042 Reactome: R-HSA-187706 Reactome: R-HSA-198203 Reactome: R-HSA-198745 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Early Endosome Membrane Late Endosome Membrane Recycling Endosome Membrane Rapidly Internalized After Ngf Binding Internalized To Endosomes Upon Binding Of Ngf Or Ntf3 And Further Transported To The Cell Body Via A Retrograde Axonal Transport Localized At Cell Membrane And Early Endosomes Before Nerve Growth Factor (Ngf) Stimulation Recruited To Late Endosomes After Ngf Stimulation Colocalized With Rapgef2 At Late Endosomes |
| Alternative Protein Names | High Affinity Nerve Growth Factor Receptor protein Neurotrophic Tyrosine Kinase Receptor Type 1 protein Trk1-Transforming Tyrosine Kinase Protein protein Tropomyosin-Related Kinase A protein Tyrosine Kinase Receptor protein Tyrosine Kinase Receptor A protein Trk-A protein Gp140trk protein P140-Trka protein NTRK1 protein MTC protein TRK protein TRKA protein |
Information sourced from Uniprot.org