• Human TLR4 (Toll-like receptor 4) CLIA Kit (STJC0000688)

Human TLR4 (Toll-like receptor 4) CLIA Kit (STJC0000688)

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STJC0000688

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human TLR4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: TLR4
Gene ID: 7099
Uniprot ID: TLR4_HUMAN
Specificity: This kit recognizes Human TLR4 in samples. No significant cross-reactivity or interference between Human TLR4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
Post Translational Modifications N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. Phosphorylated on tyrosine residues by LYN after binding lipopolysaccharide.
Function Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. At the plasma membrane, cooperates with LY96 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Mechanistically, acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Alternatively, CD14-mediated TLR4 internalization via endocytosis is associated with the initiation of a MYD88-independent signaling via the TICAM1-TBK1-IRF3 axis leading to type I interferon production. In addition to the secretion of proinflammatory cytokines, initiates the activation of NLRP3 inflammasome and formation of a positive feedback loop between autophagy and NF-kappa-B signaling cascade. In complex with TLR6, promotes inflammation in monocytes/macrophages by associating with TLR6 and the receptor CD86. Upon ligand binding, such as oxLDL or amyloid-beta 42, the TLR4:TLR6 complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway. In myeloid dendritic cells, vesicular stomatitis virus glycoprotein G but not LPS promotes the activation of IRF7, leading to type I IFN production in a CD14-dependent manner.
Protein Name Toll-Like Receptor 4
Htoll
Cd Antigen Cd284
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-140534
Reactome: R-HSA-166016
Reactome: R-HSA-166058
Reactome: R-HSA-166166
Reactome: R-HSA-2562578
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5686938
Reactome: R-HSA-936964
Reactome: R-HSA-937041
Reactome: R-HSA-937072
Reactome: R-HSA-9707616
Reactome: R-HSA-975163
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Early Endosome
Cell Projection
Ruffle
Upon Complex Formation With Cd36 And Tlr6
Internalized Through Dynamin-Dependent Endocytosis
Colocalizes With Rftn1 At Cell Membrane And Then Together With Rftn1 Moves To Endosomes
Upon Lipopolysaccharide Stimulation
Alternative CLIA Names Toll-Like Receptor 4 CLIA kit
Htoll CLIA kit
Cd Antigen Cd284 CLIA kit
TLR4 CLIA kit
Specificity This kit recognizes Human TLR4 in samples. No significant cross-reactivity or interference between Human TLR4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-97 91
EDTA plasma(n=8) 101-116 107
Cell culture media(n=8) 99-112 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 193.73 335.07 1584.16 207.30 367.47 1733.16
Standard deviation 20.79 30.79 127.52 21.25 40.16 191.86
CV (%) 10.73 9.19 8.05 10.25 10.93 11.07
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance