• Biotinylated Human TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human TIGIT is greater than 95% as determined by SEC-HPLC.
  • Immobilized Biotinylated Human TIGIT, His Tag at 0. 5 Mu g/ml (100 Mu l/well) on the streptavidin precoated plate (5 Mu g/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 8. 7ng/ml determined by ELISA.

Human TIGIT protein (Recombinant) (C-His-Avi) {Biotin} (STJP001722)

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STJP001722

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human TIGIT-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: TIGIT
Gene ID: 201633
Uniprot ID: TIGIT_HUMAN
Immunogen Region: Met22-Pro141
Immunogen: DNA sequence encoding Human TIGIT including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Function Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway. Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity.
Protein Name T-Cell Immunoreceptor With Ig And Itim Domains
V-Set And Immunoglobulin Domain-Containing Protein 9
V-Set And Transmembrane Domain-Containing Protein 3
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Clustered To The Immunological Synapse Where It Disrupts Granule Polarization And Cytotoxicity Of Nk Cells Once Engaged With Pvr
Alternative Protein Names T-Cell Immunoreceptor With Ig And Itim Domains protein
V-Set And Immunoglobulin Domain-Containing Protein 9 protein
V-Set And Transmembrane Domain-Containing Protein 3 protein
TIGIT protein
VSIG9 protein
VSTM3 protein

Information sourced from Uniprot.org

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