| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human RBX1-N-GST & C-His protein was developed from e. coli and has a target region of N-GST & C-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | RBX1 |
| Gene ID: | 9978 |
| Uniprot ID: | RBX1_HUMAN |
| Immunogen Region: | Ala2-Arg46 |
| Immunogen: | Homo sapiens (Human) |
| Function | E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Core component of a Cul9-RING ubiquitin ligase complex composed of CUL9 and RBX1, which mediates mono-ubiquitination of p53/TP53. Recruits the E2 ubiquitin-conjugating enzyme CDC34 to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. As part of a multisubunit complex composed of elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1 and CUL5.polyubiquitinates monoubiquitinated POLR2A. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rbx1E3 Ubiquitin-Protein Transferase Rbx1Protein ZypRing Finger Protein 75Ring-Box Protein 1Rbx1Regulator Of Cullins 1Roc1 Cleaved Into - E3 Ubiquitin-Protein Ligase Rbx1 - N-Terminally ProcessedE3 Ubiquitin-Protein Transferase Rbx1 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-1170546Reactome: R-HSA-1234176Reactome: R-HSA-180585Reactome: R-HSA-195253Reactome: R-HSA-2122947Reactome: R-HSA-2644606Reactome: R-HSA-2644607Reactome: R-HSA-2894862Reactome: R-HSA-4641258Reactome: R-HSA-5610780Reactome: R-HSA-5610783Reactome: R-HSA-5610785Reactome: R-HSA-5632684Reactome: R-HSA-5658442Reactome: R-HSA-5696394Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-68949Reactome: R-HSA-8854050Reactome: R-HSA-8939902Reactome: R-HSA-8951664Reactome: R-HSA-9010553Reactome: R-HSA-9020702Reactome: R-HSA-9604323Reactome: R-HSA-9679191Reactome: R-HSA-9708530Reactome: R-HSA-9725371Reactome: R-HSA-9755511Reactome: R-HSA-9762114Reactome: R-HSA-983168Reactome: R-HSA-9833109 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Protein Names | E3 Ubiquitin-Protein Ligase Rbx1 proteinE3 Ubiquitin-Protein Transferase Rbx1 proteinProtein Zyp proteinRing Finger Protein 75 proteinRing-Box Protein 1 proteinRbx1 proteinRegulator Of Cullins 1 proteinRoc1 Cleaved Into - E3 Ubiquitin-Protein Ligase Rbx1 - N-Terminally Processed proteinE3 Ubiquitin-Protein Transferase Rbx1 - N-Terminally Processed proteinRBX1 proteinRNF75 proteinROC1 protein |
Information sourced from Uniprot.org