| Host: |
CHO cells |
| Applications: |
ELISA/WB |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Growth hormone receptor (GHR)-protein was developed from cho cells. For use in research applications. |
| Conjugation: |
Biotin |
| Formulation: |
Lyophilised from 0.2 Mu m filtered 20mM Tris, 50mM NaCl, pH 7.4. |
| Dilution Range: |
Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Gene Symbol: |
GHR |
| Gene ID: |
2690 |
| Uniprot ID: |
GHR_HUMAN |
| Immunogen Region: |
ECD |
| Immunogen: |
DNA sequence encoding extracellular domain of Human GH receptor to a C-terminal polyHis tag was expressed in CHO cells. NA |
| Tissue Specificity | Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 2: Isoform 2 is expressed in lung, stomach and muscle. Isoform 4: Predominantly expressed in kidney, bladder, adrenal gland and brain stem. Highly expressed in placental villi. |
| Post Translational Modifications | The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17. On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome. Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of growth hormone receptor signaling. Ubiquitination is not sufficient for GHR internalization. |
| Function | Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Growth hormone-binding protein: The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2: Up-regulates the production of the soluble Growth hormone-binding protein form (GHBP) and acts as a negative inhibitor of growth hormone signaling. |
| Protein Name | Growth Hormone ReceptorGh ReceptorSomatotropin Receptor Cleaved Into - Growth Hormone-Binding ProteinGh-Binding ProteinGhbpSerum-Binding Protein |
| Database Links | Reactome: R-HSA-1170546Reactome: R-HSA-982772 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinOn Growth Hormone BindingGhr Is UbiquitinatedInternalizedDown-Regulated And Transported Into A Degradative Or Non-Degradative PathwayIsoform 2: Cell MembraneRemains Fixed To The Cell Membrane And Is Not InternalizedGrowth Hormone-Binding Protein: SecretedComplexed To A Substantial Fraction Of Circulating Gh |
| Alternative Protein Names | Growth Hormone Receptor proteinGh Receptor proteinSomatotropin Receptor Cleaved Into - Growth Hormone-Binding Protein proteinGh-Binding Protein proteinGhbp proteinSerum-Binding Protein proteinGHR protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
