| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
14.06pg/mL |
| Detection Limit: |
23.44~1500pg/mL |
| Short Description: |
This GDF15 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
GDF15 |
| Gene ID: |
9518 |
| Uniprot ID: |
GDF15_HUMAN |
| Specificity: |
This kit recognizes Human GDF15 in samples. No significant cross-reactivity or interference between Human GDF15 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Hormone produced in response to various stresses to confer information about those stresses to the brain, and trigger an aversive response, characterized by nausea, vomiting, and/or loss of appetite. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. Acts by binding to its receptor, GFRAL, activating GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes responses to stressful conditions. The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues. Required for avoidance behavior in response to food allergens: induced downstream of mast cell activation to promote aversion and minimize harmful effects of exposure to noxious substances. In addition to suppress appetite, also promotes weight loss by enhancing energy expenditure in muscle: acts by increasing calcium futile cycling in muscle. Contributes to the effect of metformin, an anti-diabetic drug, on appetite reduction and weight loss: produced in the kidney in response to metformin treatment, thereby activating the GDF15-GFRAL response, leading to reduced appetite and weight. The contribution of GDF15 to weight loss following metformin treatment is however limited and subject to discussion. Produced in response to anticancer drugs, such as camptothecin or cisplatin, promoting nausea, vomiting and contributing to malnutrition. Overproduced in many cancers, promoting anorexia in cancer (cachexia). Responsible for the risk of nausea and vomiting during pregnancy: high levels of GDF15 during pregnancy, mostly originating from the fetus, are associated with increased nausea and vomiting. Maternal sensitivity to nausea is probably determined by pre-pregnancy exposure to GDF15, women with naturally high level of GDF15 being less susceptible to nausea than women with low levels of GDF15 before pregnancy. Promotes metabolic adaptation in response to systemic inflammation caused by bacterial and viral infections in order to promote tissue tolerance and prevent tissue damage. Required for tissue tolerance in response to myocardial infarction by acting as an inhibitor of leukocyte integring activation, thereby protecting against cardiac rupture. Inhibits growth hormone signaling on hepatocytes. |
| Protein Name | Growth/Differentiation Factor 15Gdf-15Macrophage Inhibitory Cytokine 1Mic-1Nsaid-Activated Gene 1 ProteinNag-1Nsaid-Regulated Gene 1 ProteinNrg-1Placental Tgf-BetaPlacental Bone Morphogenetic ProteinProstate Differentiation Factor |
| Cellular Localisation | SecretedSecreted In The Plasma |
| Alternative ELISA Names | Growth/Differentiation Factor 15 ELISA kitGdf-15 ELISA kitMacrophage Inhibitory Cytokine 1 ELISA kitMic-1 ELISA kitNsaid-Activated Gene 1 Protein ELISA kitNag-1 ELISA kitNsaid-Regulated Gene 1 Protein ELISA kitNrg-1 ELISA kitPlacental Tgf-Beta ELISA kitPlacental Bone Morphogenetic Protein ELISA kitProstate Differentiation Factor ELISA kitGDF15 ELISA kitMIC1 ELISA kitPDF ELISA kitPLAB ELISA kitPTGFB ELISA kit |
| Specificity | This kit recognizes Human GDF15 in samples. No significant cross-reactivity or interference between Human GDF15 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-103 | 96 |
| EDTA plasma (n=8) | 87-102 | 94 |
| Cell culture media (n=8) | 88-100 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 83.30 | 126.61 | 615.36 | 76.84 | 120.76 | 573.31 |
| Standard deviation | 4.87 | 6.79 | 32.00 | 4.78 | 6.96 | 27.46 |
| CV (%) | 5.85 | 5.36 | 5.20 | 6.22 | 5.76 | 4.79 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
