| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.61pg/mL |
| Detection Limit: | 1.56-100pg/mL |
| Short Description : | This FGF1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | FGF1 |
| Gene ID: | 2246 |
| Uniprot ID: | FGF1_HUMAN |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. |
| Post Translational Modifications | In the nucleus, phosphorylated by PKC/PRKCD. |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis. |
| Protein Name | Fibroblast Growth Factor 1 Fgf-1 Acidic Fibroblast Growth Factor Afgf Endothelial Cell Growth Factor Ecgf Heparin-Binding Growth Factor 1 Hbgf-1 |
| Database Links | Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-1839122 Reactome: R-HSA-1839130 Reactome: R-HSA-190322 Reactome: R-HSA-190370 Reactome: R-HSA-190371 Reactome: R-HSA-190372 Reactome: R-HSA-190373 Reactome: R-HSA-190375 Reactome: R-HSA-190377 Reactome: R-HSA-2033519 Reactome: R-HSA-2219530 Reactome: R-HSA-5654219 Reactome: R-HSA-5654221 Reactome: R-HSA-5654227 Reactome: R-HSA-5654228 Reactome: R-HSA-5654687 Reactome: R-HSA-5654688 Reactome: R-HSA-5654689 Reactome: R-HSA-5654693 Reactome: R-HSA-5654695 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654704 Reactome: R-HSA-5654706 Reactome: R-HSA-5654710 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5654720 Reactome: R-HSA-5654726 Reactome: R-HSA-5654727 Reactome: R-HSA-5654732 Reactome: R-HSA-5654733 Reactome: R-HSA-5655253 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-8851708 |
| Cellular Localisation | Secreted Cytoplasm Cell Cortex Cytosol Nucleus Lacks A Cleavable Signal Sequence Within The Cytoplasm It Is Transported To The Cell Membrane And Then Secreted By A Non-Classical Pathway That Requires Cu(2+) Ions And S100a13 Secreted In A Complex With Syt1 Binding Of Exogenous Fgf1 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf1 Across Endosomal Membrane Into The Cytosol Nuclear Import From The Cytosol Requires The Classical Nuclear Import Machinery Involving Proteins Kpna1 And Kpnb1 As Well As Lrrc59 |
| Alternative ELISA Names | Fibroblast Growth Factor 1 ELISA kit Fgf-1 ELISA kit Acidic Fibroblast Growth Factor ELISA kit Afgf ELISA kit Endothelial Cell Growth Factor ELISA kit Ecgf ELISA kit Heparin-Binding Growth Factor 1 ELISA kit Hbgf-1 ELISA kit FGF1 ELISA kit FGFA ELISA kit |
| output |
Information sourced from Uniprot.org