• Human F9 (Coagulation factor IX) CLIA Kit (STJC0000393)

Human F9 (Coagulation factor IX) CLIA Kit (STJC0000393)

SKU:
STJC0000393

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This human F9 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: F9
Gene ID: 2158
Uniprot ID: FA9_HUMAN
Specificity: This kit recognizes Human F9 in samples. No significant cross-reactivity or interference between Human F9 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Detected in blood plasma (at protein level). Synthesized primarily in the liver and secreted in plasma.
Post Translational Modifications Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Function Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
Protein Name Coagulation Factor Ix
Christmas Factor
Plasma Thromboplastin Component
Ptc Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain
Database Links Reactome: R-HSA-140834
Reactome: R-HSA-140837
Reactome: R-HSA-159740
Reactome: R-HSA-159763
Reactome: R-HSA-159782
Reactome: R-HSA-9672383
Reactome: R-HSA-9672396
Reactome: R-HSA-9673202
Reactome: R-HSA-9673218
Reactome: R-HSA-9673221
Reactome: R-HSA-9673240
Cellular Localisation Secreted
Alternative CLIA Names Coagulation Factor Ix CLIA kit
Christmas Factor CLIA kit
Plasma Thromboplastin Component CLIA kit
Ptc Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain CLIA kit
F9 CLIA kit
Specificity This kit recognizes Human F9 in samples. No significant cross-reactivity or interference between Human F9 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 99
EDTA plasma(n=8) 84-98 91
Cell culture media(n=8) 93-109 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.96 1.99 8.48 0.97 2.09 8.92
Standard deviation 0.11 0.20 0.68 0.11 0.18 1.01
CV (%) 11.46 10.05 8.02 11.34 8.61 11.32
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance