| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1.0 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized lpilimumab at 1 Mu g/mL (100 Mu L/well) can bind Recombinant Human CTLA-4 with a linear range of 6-18 ng/mL.|2.Measured by its binding ability in a functional ELISA.Immobilized PE ant |
| Gene Symbol: | CTLA4 |
| Gene ID: | 1493 |
| Uniprot ID: | CTLA4_HUMAN |
| Immunogen Region: | Ala37-Phe162 |
| Immunogen: | Recombinant Human CTLA-4/CD152 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala37-Phe162) of human CTLA-4 (Accession #NP_005205.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | AMHVAQPAVVLASSRGIASF VCEYASPGKATEVRVTVLRQ ADSQVTEVCAATYMMGNELT FLDDSICTGTSSGNQVNLTI QGLRAMDTGLYICKVELMYP PPYYLGIGNGTQIYVIDPEP CPDSDF |
| Post Translational Modifications | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
| Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Protein Name | Cytotoxic T-Lymphocyte Protein 4Cytotoxic T-Lymphocyte-Associated Antigen 4Ctla-4Cd Antigen Cd152 |
| Database Links | Reactome: R-HSA-389513Reactome: R-HSA-8877330 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization |
| Alternative Protein Names | Cytotoxic T-Lymphocyte Protein 4 proteinCytotoxic T-Lymphocyte-Associated Antigen 4 proteinCtla-4 proteinCd Antigen Cd152 proteinCTLA4 proteinCD152 protein |
Information sourced from Uniprot.org