GPR56 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:500-1:750
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Widely distributed with highest levels found in thyroid gland, brain and heart. Expressed in a great number of tumor cells. Expression is down-regulated in different tumors from highly metastatic cells.
Post Translational Modifications
Autoproteolytically cleaved into 2 fragments.the large extracellular N-terminal fragment (ADGRG1 NT) and the membrane-bound C-terminal fragment (ADGRG1 CT) predominantly remain associated and non-covalently linked. Shedding to yield the secreted ADGRG1 N-terminal fragment seems to involve metalloprotease(s). N-glycosylated. Contains sialic acid residues. Ubiquitinated. Undergoes polyubiquitination upon activation.
Function
Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination. Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12. Plays a role in the maintenance of hematopoietic stem cells and/or leukemia stem cells in bone marrow niche. Plays a critical role in cancer progression by inhibiting VEGFA production threreby inhibiting angiogenesis through a signaling pathway mediated by PRKCA. Plays an essential role in testis development. ADGRG1 N-terminal fragment: Plays a critical role in cancer progression by activating VEGFA production and angiogenesis through a signaling pathway mediated by PRKCA.
Cell MembraneMulti-Pass Membrane ProteinAdgrg1 N-Terminal Fragment: SecretedAdgrg1 C-Terminal Fragment: Membrane RaftInteraction With Its Ligand Col3a1 Leads To The Release Of Adgrg1 Nt From The Membrane And Triggers The Association Of Adgrg1 Ct With Lipid Rafts
Alternative Peptide Names
Adhesion G-Protein Coupled Receptor G1 proteinG-Protein Coupled Receptor 56 proteinProtein Tm7xn1 Cleaved Into - Adgrg1 N-Terminal Fragment proteinAdgrg1 Nt proteinGpr56 N-Terminal Fragment proteinGpr56 Nt proteinGpr56(N proteinGpr56 Extracellular Subunit proteinGpr56 Subunit Alpha - Adgrg1 C-Terminal Fragment proteinAdgrg1 Ct proteinGpr56 C-Terminal Fragment proteinGpr56 Ct proteinGpr56(C proteinGpr56 Seven-Transmembrane Subunit proteinGpr56 7tm proteinGpr56 Subunit Beta proteinADGRG1 proteinGPR56 proteinTM7LN4 proteinTM7XN1 proteinUNQ540 proteinPRO1083 protein
