| Applications: | Immunodepletion/Immunocompetition |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Phospho-Filamin B Blocking Peptide is synthetically produced from the 2450-2500 sequence about the 2478 and is suitable for use in western blot applications. |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | FLNB |
| Gene ID: | 2317 |
| Uniprot ID: | FLNB_HUMAN |
| Immunogen Region: | 2450-2500 |
| Immunogen: | Synthetic peptide taken within amino acid region 2450-2500 on human Phospho-Filamin-B. Phosphorylated 2478 serine residue. |
| Tissue Specificity | Ubiquitous. Isoform 1 and isoform 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells (HUVEC), retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord, platelet and Daudi cells. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer disease (AD). Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart. |
| Post Translational Modifications | ISGylation prevents ability to interact with the upstream activators of the JNK cascade and inhibits IFNA-induced JNK signaling. Ubiquitination by a SCF-like complex containing ASB2 isoform 1 leads to proteasomal degradation which promotes muscle differentiation. |
| Function | Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro. |
| Peptide Name | Filamin-B Fln-B Abp-278 Abp-280 Homolog Actin-Binding-Like Protein Beta-Filamin Filamin Homolog 1 Fh1 Filamin-3 Thyroid Autoantigen Truncated Actin-Binding Protein Truncated Abp |
| Database Links | Reactome: R-HSA-1169408 |
| Cellular Localisation | Isoform 1: Cytoplasm Cell Cortex Cytoplasm Cytoskeleton Stress Fiber Myofibril Sarcomere Z Line In Differentiating Myotubes Isoform 1 Isoform 2 And Isoform 3 Are Localized Diffusely Throughout The Cytoplasm With Regions Of Enrichment At The Longitudinal Actin Stress Fiber In Differentiated Tubes Isoform 1 Is Also Detected Within The Z-Lines Isoform 2: Cytoplasm Isoform 3: Cytoplasm Isoform 6: Cytoplasm Polarized At The Periphery Of Myotubes |
| Alternative Peptide Names | Filamin-B protein Fln-B protein Abp-278 protein Abp-280 Homolog protein Actin-Binding-Like Protein protein Beta-Filamin protein Filamin Homolog 1 protein Fh1 protein Filamin-3 protein Thyroid Autoantigen protein Truncated Actin-Binding Protein protein Truncated Abp protein FLNB protein FLN1L protein FLN3 protein TABP protein TAP protein |
Information sourced from Uniprot.org