| Applications: | Immunodepletion/Immunocompetition |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | FGR Blocking Peptide is synthetically produced from the 450-443 sequence and is suitable for use in western blot applications. |
| Formulation: | Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | FGR |
| Gene ID: | 2268 |
| Uniprot ID: | FGR_HUMAN |
| Immunogen Region: | 450-443 |
| Immunogen: | Synthetic peptide taken within amino acid region 450-443 on human Fgr protein. |
| Post Translational Modifications | Ubiquitinated. Becomes ubiquitinated in response to ITGB2 signaling.this does not lead to degradation. Phosphorylated. Autophosphorylated on tyrosine residues. Becomes phosphorylated in response to FCGR2A and/or FCGR2B engagement, cell adhesion and signaling by ITGB2. Prior phosphorylation at Tyr-523 by SRC inhibits ulterior autophosphorylation at Tyr-412. |
| Function | Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as a negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as a positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1. Together with CLNK, it acts as a negative regulator of natural killer cell-activating receptors and inhibits interferon-gamma production. |
| Peptide Name | Tyrosine-Protein Kinase Fgr Gardner-Rasheed Feline Sarcoma Viral V-Fgr Oncogene Homolog Proto-Oncogene C-Fgr P55-Fgr P58-Fgr P58c-Fgr |
| Database Links | Reactome: R-HSA-2029481 Reactome: R-HSA-432142 Reactome: R-HSA-6798695 Reactome: R-HSA-9664323 Reactome: R-HSA-9664422 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Cytoplasmic Side Peripheral Membrane Protein Cell Projection Ruffle Membrane Cytoplasm Cytosol Cytoskeleton Mitochondrion Inner Membrane Mitochondrion Intermembrane Space Detected In Mitochondrial Intermembrane Space And At Inner Membranes Colocalizes With Actin Fibers At Membrane Ruffles Detected At Plasma Membrane Lipid Rafts |
| Alternative Peptide Names | Tyrosine-Protein Kinase Fgr protein Gardner-Rasheed Feline Sarcoma Viral protein V-Fgr Oncogene Homolog protein Proto-Oncogene C-Fgr protein P55-Fgr protein P58-Fgr protein P58c-Fgr protein FGR protein SRC2 protein |
Information sourced from Uniprot.org