Posted by M.Popov on 12th Nov 2020
Trial size GAPDH antibodies available in the Antibody Validation Project
Trial size GAPDH antibodies available in the Antibody Validation Project
Abstract:
- What is GAPDH?
- Where is GAPDH found?
- What role does GAPDH play in disease?
- GAPDH antibodies in the Antibody Validation project
- Customer reviewed GAPDH antibodies
- Other GAPDH antibodies available in the Antibody Validation Project
- Popular GAPDH antibodies not currently in the Antibody Validation Project
- How does the Antibody Validation Project work?
What is GAPDH?
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a housekeping gene taking part in glycolysis. It is a primary cytoplasmic protein and an essential metabolic regulator. It has been proven that GAPDH is involved in many cellular processes such as membrane fusion, DNA repair and cell death.
Where is GAPDH found?
Being a cytoplasmic protein doesn't limit its spread to just the cytoplasm. GAPDH can also be found in the nucleus, small vesicular fractions and the mitochondria. Under stress exposure, GAPDH dynamically relocates and distributes on a subcellular level.
What role does GAPDH play in disease?
Being a initiator of cell death, GAPDH is present in large nuclear quantities in patients suffering from neurodegenerative conditions, such as Alzheimer's, Parkinson's, Huntington's and glaucoma. Apart from that, GAPDH is often used as a loading control for western blot and also as control for qPCR. This is possible because the gene is mostly stably and expressed at high levels in the majority of the cells and tissues. Under specific conditions, regulations for GAPDH have been found. Because of that, it is really important to carefully consider if using GAPDH as a loading control is suitable or not.
GAPDH antibodies in the Antibody Validation project
St John's Laboratory offers a variety of trial size primary antibodies which target GAPDH, which are available to try through the Antibody Validation Project. Below we highlight those which have already been reviewed, as well as others which are available to try.
Customer reviewed GAPDH antibodies
| Model | Name | Host | Clonality | Rating | Application Tested |
|---|---|---|---|---|---|
| STJ96931 | Anti-GAPDH antibody | Mouse | Monoclonal | 5 | WB |
| STJ97090 | Anti-GAPDH antibody | Rabbit | Polyclonal | 5 |
Other GAPDH antibodies available in the Antibody Validation Project
| Model | Name | Host | Clonality | Recommended Applications |
|---|---|---|---|---|
| STJ140037 | Anti-GAPDH antibody | Goat | Polyclonal | WB,IF,IHC |
| STJ140038 | Anti-GAPDH antibody | Goat | Polyclonal | WB,IF,IHC |
| STJ96942 | Anti-GAPDH antibody | Mouse | Monoclonal | WB |
| STJ97090 | Anti-GAPDH antibody | Rabbit | Polyclonal | WB,IHC |
| STJ97102 | Anti-GAPDH antibody | Rabbit | Polyclonal | WB |
| STJ97462 | Anti-GAPDH antibody | Rabbit | Polyclonal | WB,IHC |
| STJ98096 | Anti-GAPDH antibody | Mouse | Monoclonal | WB,IHC,IF,ELISA |
| STJ98494 | Anti-GAPDH antibody | Mouse | Monoclonal | WB |
| STJ99065 | Anti-GAPDH (Human Specific) antibody | Mouse | Monoclonal | WB,ELISA |
| STJ99066 | Anti-GAPDH antibody | Mouse | Monoclonal | WB,ELISA |
Popular GAPDH antibodies not currently in the Antibody Validation Project
| Model | Name | Host | Clonality | Recommended Applications |
|---|---|---|---|---|
| STJ112764 | Anti-GAPDH Antibody | Rabbit | Monoclonal | WB,IHC,IF,IP |
| STJ70553 | Anti-GAPDH Antibody | Goat | Polyclonal | ELISA,WB,IF,IHC |
| STJ70842 | Anti-GAPDH Antibody | Goat | Polyclonal | ELISA,WB,IHC,IF |
| STJ73172 | Anti-GAPDH Antibody (Biotin) | Goat | Polyclonal | ELISA,WB,IHC |
| STJ73275 | Anti-GAPDH Antibody (Biotin) | Goat | Polyclonal | ELISA,WB |
How does the Antibody Validation Project work?
In order to help our customers with their choice of antibody with low risk, we offer one of the largest ranges of primary antibodies with trial size options available.
The Antibody Validation Project works like this.
1. Order your trial size antibody online.
2. Explore its suitability.
3. Return your results by posting them on the product page.
4. Receive a sample refund.
Find out more here.