Control and blocking peptides for your immunoassay experiments

Ensure antibody specificity and assay reliability with blocking and control peptides from St John's Laboratory. Blocking peptides confirm your antibody's target accuracy by eliminating off-target binding, while control peptides validate your assay's performance by confirming successful epitope recognition. Together, they strengthen confidence in your ELISA, Western blot, or IHC results.

    Purchase blocking peptides with confidence

    Eliminate doubt. Confirm specificity. Get results you can trust.

      Designed to enhance the specificity of your immunoassay experiments, St John’s Laboratory offers a range of high-purity control and blocking peptides to support confident antibody validation in Western blot, IHC, and ELISA.

      • Validate Antibody Specificity : Pre-incubate your antibody with a matching blocking peptide to confirm it binds only to your target protein.
      • High Purity & Epitope Precision : Synthesised to mirror your antigen’s binding site with exact accuracy.
      • Reduce Background & False Positives : Distinguish specific vs. non-specific signal with precision controls.
      • Rigorous Batch QC : Every peptide is tested for consistency and performance to ensure reproducible results.

      Ready to improve your assay reliability? Browse our full catalogue of blocking and positive control peptides today.

      Our blocking peptide products

      Confirm antibody specificity with St John's Laboratory's high-purity blocking peptides. Essential for immunodepletion and immunocompetition

      Blocking peptide targeting GPCRs

      The 5HT1A antibody specifically targets the 5HT1A receptor, which is crucial for serotonin signaling and neurological research. The 5HT1A Blocking Peptide serves as a valuable control for antibody specificity in Western blotting (WB) and IHC and competes with or completely blocks the antibody, preventing it from binding to the receptor in experiments.

      Blocking peptides targeting tyrosine kinases

      The ABL1 antibody detects the ABL1 kinase, which is vital for cell growth and a key target in cancer research, especially leukemia. Use ABL1 Blocking Peptide to validate your results.

      Blocking peptide targeting neurological proteins

      To effectively investigate alpha synuclein in neurological conditions like synucleinopathies, precise tools are essential. Alpha synuclein blocking peptide (STJ504398) is specifically designed to be used with an alpha synuclein antibody. Enhance the specificity and reliability of your experiments by confirming accurate antibody binding to alpha synuclein—a crucial factor in synaptic function and neurodegeneration. By using this control, you can enhance the accuracy of your findings and accelerate your neuroscience research.

      Browse our blocking peptides

      Positive Control Peptides: Essential controls for achieving accurate results

      Our positive control peptides are indispensable for validating assay performance.

        A positive control peptide contains the immunogen sequence for the antibody you are using. Positive control peptides:

      • Provide robust antibody validation: Validate the specificity of your antibody to ensure it binds to its intended target and not to off-target proteins.
      • Guarantee accurate data interpretation for your Western blot, IHC or ELISA assays: Have confidence in your results by specifically depleting an antibody's activity or competing for binding sites, providing definitive proof of specific antibody-antigen interaction in assays.

        • Positive controls are critical benchmarks in experiments, ensuring the entire procedure functions correctly and is optimised. A positive outcome confirms the reliability of the method, validating that any negative results are authentic. A positive outcome from the positive control indicates that the procedure is functioning correctly and has been optimised. This ensures that any negative results are authentic.

        How do positive control peptides work? For direct ELISA: The positive control peptide is directly coated onto the wells of an ELISA plate. The antibody is then added, and its binding is detected. Successful antibody binding to the peptide directly confirms the antibody's ability to recognise and bind to its specific epitope.

        Positive controls

        FAQs

        What are Blocking Peptides? Also known as "immunising peptides" or "negative control antigens", a blocking peptide is a synthetic replica of a protein's epitope. In the presence of a blocking peptide, an antibody is no longer able to bind to an epitope present in a protein. They provide valuable tools to validate antibody-antigen interactions. A positive control peptide contains the immunogen sequence for the antibody you are using.

        Why are positive controls essential? Positive controls are critical benchmarks in experiments, ensuring the entire procedure functions correctly and is optimised. A positive outcome confirms the reliability of the method, validating that any negative results are authentic. A positive outcome from the positive control indicates that the procedure is functioning correctly and has been optimised. This ensures that any negative results are authentic.

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