• Human tonsil stained with anti-ZAP-70 antibodies using peroxidase-conjugate and DAB chromogen. Note the positive staining of perifollicular B-cells

Anti-ZAP70 antibody (247-382aa) [ZM97] (STJ180332)

SKU:
STJ180332

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Host: Mouse
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-ZAP70 (247-382aa) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: ZM97
Conjugation: Unconjugated
Isotype: IgG1/Kappa
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:100‐200
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: ZAP70
Gene ID: 7535
Uniprot ID: ZAP70_HUMAN
Immunogen Region: 247-382aa
Specificity: Positive control: Tonsil, CLL/SLL
Immunogen: Recombinant fragment of human ZAP70 protein (around aa247-382)
Tissue Specificity Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
Post Translational Modifications Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22. Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B.
Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
Protein Name Tyrosine-Protein Kinase Zap-70
70 Kda Zeta-Chain Associated Protein
Syk-Related Tyrosine Kinase
Database Links Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-9013407
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
In Quiescent T-Lymphocytes
It Is Cytoplasmic
Upon Tcr Activation
It Is Recruited At The Plasma Membrane By Interacting With Cd247/Cd3z
Colocalizes Together With Rhoh In The Immunological Synapse
Rhoh Is Required For Its Proper Localization To The Cell Membrane And Cytoskeleton Fractions In The Thymocytes
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Zap-70 antibody
Anti-70 Kda Zeta-Chain Associated Protein antibody
Anti-Syk-Related Tyrosine Kinase antibody
Anti-ZAP70 antibody
Anti-SRK antibody

Information sourced from Uniprot.org

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