• Immunohistochemistry analysis of USP47 in paraffin-embedded Human colon carcinoma using USP47 Rabbit polyclonal antibody (STJ117656) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using USP47 Rabbit polyclonal antibody (STJ117656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-USP47 antibody (1128-1287) (STJ117656)

SKU:
STJ117656

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP47 (1128-1287) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP47
Gene ID: 55031
Uniprot ID: UBP47_HUMAN
Immunogen Region: 1128-1287
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1128-1287 of human USP47 (NP_060414.3).
Immunogen Sequence: VEKMKSMSQLAVLSRRWKPS EMKLDPFQEVVLESSSVDEL REKLSEISGIPLDDIEFAKG RGTFPCDISVLDIHQDLDWN PKVSTLNVWPLYICDDGAVI FYRDKTEELMELTDEQRNEL MKKESSRLQKTGHRVTYSPR KEKALKIYLDGAPNKDLTQD
Tissue Specificity Expressed in skeletal muscle, heart and testis.
Function Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 47
Deubiquitinating Enzyme 47
Ubiquitin Thioesterase 47
Ubiquitin-Specific-Processing Protease 47
Database Links Reactome: R-HSA-5689880
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 47 antibody
Anti-Deubiquitinating Enzyme 47 antibody
Anti-Ubiquitin Thioesterase 47 antibody
Anti-Ubiquitin-Specific-Processing Protease 47 antibody
Anti-USP47 antibody

Information sourced from Uniprot.org

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