| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Unconventional prefoldin RPB5 interactor 1 (415-464 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:40000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | URI1 |
| Gene ID: | 8725 |
| Uniprot ID: | RMP_HUMAN |
| Immunogen Region: | 415-464 aa |
| Specificity: | RMP Polyclonal Antibody detects endogenous levels of RMP protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human RMP at the amino acid range 415-464 |
| Post Translational Modifications | Phosphorylated. Phosphorylation occurs essentially on serine residues. Phosphorylation occurs in response to androgen treatment in prostate cancer cells in a mTOR-dependent manner. Phosphorylated.hyperhosphorylated in mitochondria in a mTORC-dependent signaling pathway. Phosphorylated at Ser-372 by RPS6KB1 in a growth factor- and rapamycin-dependent manner. S6K1-mediated mitochondrial phosphorylation at Ser-372 disrupts the URI1-PPP1CC complex in the mitochondrion, relieves PPP1CC phosphatase inhibition activity and hence engages a negative feedback diminishing RPS6KB1 kinase activity, preventing sustained S6K1-dependent signaling. |
| Function | Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein. Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination. |
| Protein Name | Unconventional Prefoldin Rpb5 Interactor 1 Protein Nnx3 Protein Phosphatase 1 Regulatory Subunit 19 Rna Polymerase Ii Subunit 5-Mediating Protein Rpb5-Mediating Protein |
| Cellular Localisation | Nucleus Cytoplasm Mitochondrion Cell Projection Dendrite Colocalizes With Pfdn2 Pfdn4 Ppp1cc Rps6kb1 And Stap1 At Mitochondrion |
| Alternative Antibody Names | Anti-Unconventional Prefoldin Rpb5 Interactor 1 antibody Anti-Protein Nnx3 antibody Anti-Protein Phosphatase 1 Regulatory Subunit 19 antibody Anti-Rna Polymerase Ii Subunit 5-Mediating Protein antibody Anti-Rpb5-Mediating Protein antibody Anti-URI1 antibody Anti-C19orf2 antibody Anti-NNX3 antibody Anti-PPP1R19 antibody Anti-RMP antibody Anti-URI antibody |
Information sourced from Uniprot.org