Western blot analysis of extracts of various cell lines, using UPK1A antibody (STJ114634) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-UPK1A antibody (110-230) (STJ114634)

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STJ114634
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UPK1A
Gene ID: 11045
Uniprot ID: UPK1A_HUMAN
Immunogen Region: 110-230
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 110-230 of human UPK1A (NP_008931.1).
Immunogen Sequence: SCITSYTHRDYMVSNPSLIT KQMLTFYSADTDQGQELTRL WDRVMIEQECCGTSGPMDWV NFTSAFRAATPEVVFPWPPL CCRRTGNFIPLNEEGCRLGH MDYLFTKGCFEHIGHAIDSY T
Tissue Specificity High expression restricted to ureteric urothelium (most superficial cells).low expression in prostate. Expression in normal urothelial cells is lost in culture. Some expression in tumor cell lines derived from urothelial malignancies.
Function Component of the asymmetric unit membrane (AUM).a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.
Protein Name Uroplakin-1a
Up1a
Tetraspanin-21
Tspan-21
Uroplakin Ia
Upia
Upka
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Uroplakin-1a antibody
Anti-Up1a antibody
Anti-Tetraspanin-21 antibody
Anti-Tspan-21 antibody
Anti-Uroplakin Ia antibody
Anti-Upia antibody
Anti-Upka antibody
Anti-UPK1A antibody
Anti-TSPAN21 antibody

Information sourced from Uniprot.org

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