| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UBR4 |
| Gene ID: |
23352 |
| Uniprot ID: |
UBR4_HUMAN |
| Immunogen Region: |
4884-5183 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 4884-5183 of human UBR4 (NP_065816.2). |
| Immunogen Sequence: |
NIVHYDCHLAAVRLARGREE WESAALQNANTKCNGLLPVW GPHVPESAFATCLARHNTYL QECTGQREPTYQLNIHDIKL LFLRFAMEQSFSADTGGGGR ESNIHLIPYIIHTVLYVLNT TRATSREEKNLQGFLEQPKE KWVESAFEVDGPYYFTVLAL HILPPEQWRATRVEILRRLL VTSQARAVAPGGATRLTDKA VKDYSAYRSSLLFWALVDLI YNMFKKVPTSNTEGGWSCS |
| Function | E3 ubiquitin-protein ligase involved in different protein quality control pathways in the cytoplasm. Component of the N-end rule pathway: ubiquitinates proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their degradation. Recognizes both type-1 and type-2 N-degrons, containing positively charged amino acids (Arg, Lys and His) and bulky and hydrophobic amino acids, respectively. Does not ubiquitinate proteins that are acetylated at the N-terminus. Together with UBR5, part of a cytoplasm protein quality control pathway that prevents protein aggregation by catalyzing assembly of heterotypic 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on aggregated proteins, leading to substrate recognition by the segregase p97/VCP and degradation by the proteasome: UBR4 probably synthesizes mixed chains containing multiple linkages, while UBR5 is likely branching multiple 'Lys-48'-linked chains of substrates initially modified. Together with KCMF1, part of a protein quality control pathway that catalyzes ubiquitination and degradation of proteins that have been oxidized in response to reactive oxygen species (ROS): recognizes proteins with an Arg-CysO3(H) degron at the N-terminus, and mediates assembly of heterotypic 'Lys-63'-/'Lys-27'-linked branched ubiquitin chains on oxidized proteins, leading to their degradation by autophagy. Catalytic component of the SIFI complex, a multiprotein complex required to inhibit the mitochondrial stress response after a specific stress event has been resolved: ubiquitinates and degrades (1) components of the HRI-mediated signaling of the integrated stress response, such as DELE1 and EIF2AK1/HRI, as well as (2) unimported mitochondrial precursors. Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1. Mediates ubiquitination of ACLY, leading to its subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. |
| Protein Name | E3 Ubiquitin-Protein Ligase Ubr4600 Kda Retinoblastoma Protein-Associated FactorP600N-Recognin-4Retinoblastoma-Associated Factor Of 600 KdaRbaf600 |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytoskeletonNucleusLocalizes To Endosomes Via Its Association With Calcium-Bound CalmodulinConcentrates At The Leading Edge Of Membrane Structures Involved In Actin Motility |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Ubr4 antibodyAnti-600 Kda Retinoblastoma Protein-Associated Factor antibodyAnti-P600 antibodyAnti-N-Recognin-4 antibodyAnti-Retinoblastoma-Associated Factor Of 600 Kda antibodyAnti-Rbaf600 antibodyAnti-UBR4 antibodyAnti-KIAA0462 antibodyAnti-KIAA1307 antibodyAnti-RBAF600 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
