| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBE3C |
| Gene ID: | 9690 |
| Uniprot ID: | UBE3C_HUMAN |
| Immunogen Region: | 1-270 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human UBE3C (NP_055486.2). |
| Immunogen Sequence: | MFSFEGDFKTRPKVSLGGAS RKEEKASLLHRTQEERRKRE EERRRLKNAIIIQSFIRGYR DRKQQYSIQRSAFDRCATLS QSGGAFPIANGPNLTLLVRQ LLFFYKQNEDSKRLIWLYQN LIKHSSLFVKQLDGSERLTC LFQIKRLMSLCCRLLQNCND DSLNVALPMRMLEVFSSENT YLPVLQDASYVVSVIEQILH YMIHNGYYRSLYLLINSKLP SSIEYSDLSRVPIAKILLE |
| Tissue Specificity | Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas. |
| Post Translational Modifications | Autoubiquitinated.promoting its own degradation. |
| Function | E3 ubiquitin-protein ligase that specifically catalyzes 'Lys-29'- and 'Lys-48'-linked polyubiquitin chains. Accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Associates with the proteasome and promotes elongation of ubiquitin chains on substrates bound to the 26S proteasome. Also catalyzes 'Lys-29'- and 'Lys-48'-linked ubiquitination of 26S proteasome subunit ADRM1/RPN13 in response to proteotoxic stress, impairing the ability of the proteasome to bind and degrade ubiquitin-conjugated proteins. Acts as a negative regulator of autophagy by mediating 'Lys-29'- and 'Lys-48'-linked ubiquitination of PIK3C3/VPS34, promoting its degradation. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains.with some preference for 'Lys-48' linkages. Acts as a negative regulator of type I interferon by mediating 'Lys-48'-linked ubiquitination of IRF3 and IRF7, leading to their degradation by the proteasome. Catalyzes ubiquitination and degradation of CAND2. |
| Protein Name | Ubiquitin-Protein Ligase E3c Hect-Type Ubiquitin Transferase E3c Homologous To E6ap Carboxyl Terminus Homologous Protein 2 Hecth2 Rta-Associated Ubiquitin Ligase Raul |
| Database Links | Reactome: R-HSA-983168 |
| Alternative Antibody Names | Anti-Ubiquitin-Protein Ligase E3c antibody Anti-Hect-Type Ubiquitin Transferase E3c antibody Anti-Homologous To E6ap Carboxyl Terminus Homologous Protein 2 antibody Anti-Hecth2 antibody Anti-Rta-Associated Ubiquitin Ligase antibody Anti-Raul antibody Anti-UBE3C antibody Anti-KIAA0010 antibody Anti-KIAA10 antibody |
Information sourced from Uniprot.org