Host: | Rabbit |
Applications: | WB/IHC |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-TSC2 (1160-1255) is suitable for use in Western Blot and Immunohistochemistry research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
Purification: | Affinity purification |
Dilution Range: | WB 1:500-1:1000 IHC-P 1:50-1:200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | TSC2 |
Gene ID: | 7249 |
Uniprot ID: | TSC2_HUMAN |
Immunogen Region: | 1160-1255 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1160-1255 of human TSC2 (NP_000539.2). |
Immunogen Sequence: | TAPAAKPEKASAGTRVPVQE KTNLAAYVPLLTQGWAEILV RRPTGNTSWLMSLENPLSPF SSDINNMPLQELSNALMAAE RFKEHRDTALYKSLSV |
Tissue Specificity | Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta. |
Post Translational Modifications | Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 in response to insulin signaling and growth factor stimulation inhibits the ability of the TSC-TBC complex to suppress mTORC1 signaling: phosphorylation promotes dissociation of the TSC-TBC complex from lysosomal membranes, leading to activation of mTORC1 by RHEB. Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1. Phosphorylation by AMPK activates it and leads to negative regulation of the mTORC1 complex. Phosphorylated at Ser-1798 by RPS6KA1.phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling. Phosphorylated by DAPK1. Ubiquitinated by the DCX(FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation. Ubiquitinated by MYCBP2 independently of its phosphorylation status leading to subsequent degradation.association with TSC1 protects from ubiquitination. |
Function | Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5. |
Protein Name | Tuberin Tuberous Sclerosis 2 Protein |
Database Links | Reactome: R-HSA-1632852 Reactome: R-HSA-165181 Reactome: R-HSA-198323 Reactome: R-HSA-380972 Reactome: R-HSA-5628897 Reactome: R-HSA-5674400 Reactome: R-HSA-8854214 |
Cellular Localisation | Lysosome Membrane Peripheral Membrane Protein Cytoplasm Cytosol Recruited To Lysosomal Membranes In A Rheb-Dependent Process In Absence Of Nutrients In Response To Insulin Signaling And Phosphorylation By Pkb/Akt1 The Complex Dissociates From Lysosomal Membranes And Relocalizes To The Cytosol |
Alternative Antibody Names | Anti-Tuberin antibody Anti-Tuberous Sclerosis 2 Protein antibody Anti-TSC2 antibody Anti-TSC4 antibody |
Information sourced from Uniprot.org