STJ70249 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-TRIM37 antibody (C-Term) (STJ70249)

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STJ70249-100
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-TRIM37 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:64000.
IF: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of HeLa cells. Recommended concentrat
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TRIM37
Gene ID: 4591
Uniprot ID: TRI37_HUMAN
Immunogen Region: C-Term
Accession Number: NP_056109.1;Isotype=IgG"
Specificity: Expercted to recognise all reported isoforms a to i
Immunogen Sequence: EDLSFNTDENSGR
Post Translational Modifications Auto-ubiquitinated.
Function E3 ubiquitin-protein ligase required to prevent centriole reduplication. Probably acts by ubiquitinating positive regulators of centriole reduplication. Mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression: associates with some Polycomb group (PcG) multiprotein PRC2-like complex and mediates repression of target genes. Also acts as a positive regulator of peroxisome import by mediating monoubiquitination of PEX5 at 'Lys-472': monoubiquitination promotes PEX5 stabilitation by preventing its polyubiquitination and degradation by the proteasome. Has anti-HIV activity.
Protein Name E3 Ubiquitin-Protein Ligase Trim37
Mulibrey Nanism Protein
Ring-Type E3 Ubiquitin Transferase Trim37
Tripartite Motif-Containing Protein 37
Database Links Reactome: R-HSA-983168
Cellular Localisation Chromosome
Cytoplasm
Perinuclear Region
Peroxisome Membrane
Peripheral Membrane Protein
Found In Vesicles Of The Peroxisome
Aggregates As Aggresomes
A Perinuclear Region Where Certain Misfolded Or Aggregated Proteins Are Sequestered For Proteasomal Degradation
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim37 antibody
Anti-Mulibrey Nanism Protein antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim37 antibody
Anti-Tripartite Motif-Containing Protein 37 antibody
Anti-TRIM37 antibody
Anti-KIAA0898 antibody
Anti-MUL antibody
Anti-POB1 antibody

Information sourced from Uniprot.org

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