| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Tri-Methyl-Histone H3.1 and Histone H3.2 and Histone H3.3 and Histone H3.3C-K5 is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 ELISA 1:20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | H3C15.H3C14.H3C13 H3-5 126961/333932/653604 440093 |
| Uniprot ID: | H32_HUMAN H3C_HUMAN H33_HUMAN < |
| Specificity: | This antibody detects endogenous levels of Histone H3 at Human:K5Mouse:K5Rat:K5, It doesn t reacte with total protein. |
| Immunogen: | Synthesized Tri-Methyl peptide derived from the human Histone H3. at the amino acid range K5 |
| Post Translational Modifications | Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity. Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK. Monoubiquitinated.monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors.results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7.deubiquitination is induced by oxidative stress.enhances its interaction with USP7 and consequently, deubiquitination.increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability. |
| Function | Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. |
| Protein Name | Forkhead Box Protein O4 Fork Head Domain Transcription Factor Afx1 |
| Database Links | Reactome: R-HSA-198693 Reactome: R-HSA-5674400 Reactome: R-HSA-5689880 Reactome: R-HSA-9614399 Reactome: R-HSA-9614657 Reactome: R-HSA-9615017 Reactome: R-HSA-9617629 Reactome: R-HSA-9617828 |
| Cellular Localisation | Cytoplasm Nucleus When Phosphorylated Translocated From Nucleus To Cytoplasm Dephosphorylation Triggers Nuclear Translocation Monoubiquitination Increases Nuclear Localization When Deubiquitinated |
| Alternative Antibody Names | Anti-Forkhead Box Protein O4 antibody Anti-Fork Head Domain Transcription Factor Afx1 antibody Anti-FOXO4 antibody Anti-AFX antibody Anti-AFX1 antibody Anti-MLLT7 antibody |
Information sourced from Uniprot.org