| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TOPBP1 (1253-1522) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TOPBP1 |
| Gene ID: |
11073 |
| Uniprot ID: |
TOPB1_HUMAN |
| Immunogen Region: |
1253-1522 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2). |
| Immunogen Sequence: |
KIITIEETHEELKKQYIFQL SSLNPQERIDYCHLIEKLGG LVIEKQCFDPTCTHIVVGHP LRNEKYLASVAAGKWVLHRS YLEACRTAGHFVQEEDYEWG SSSILDVLTGINVQQRRLAL AAMRWRKKIQQRQESGIVEG AFSGWKVILHVDQSREAGFK RLLQSGGAKVLPGHSVPLFK EATHLFSDLNKLKPDDSGVN IAEAAAQNVYCLRTEYIADY LMQESPPHVENYCLPEAIS |
| Tissue Specificity | Highly expressed in heart, brain, placenta, lung and kidney. |
| Post Translational Modifications | Phosphorylated on serine and threonine residues in response to X-ray irradiation. Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. Deubiquitinated by USP13.leading to TOPBP1 stabilizion and activation of the ATR-TOPBP1 axis pathway. |
| Function | Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. |
| Protein Name | Dna Topoisomerase 2-Binding Protein 1Dna Topoisomerase Ii-Beta-Binding Protein 1Topbp1Dna Topoisomerase Ii-Binding Protein 1 |
| Database Links | Reactome: R-HSA-5685938Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9709570 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle PoleChromosomeDetected On Unpaired Autosomes In Meiotic Prophase CellsDetected On X And Y Chromosomes During Later Stages Of ProphaseColocalizes With Atr And H2ax At Unsynapsed Chromosome Cores During ProphaseHas A Uniform Nuclear Distribution During G PhaseColocalizes With Brca1 At Stalled Replication Forks During S PhaseIn Mitotic Cells It Colocalizes With Brca1 At Spindle Poles And Centrosomes During Metaphase And AnaphaseDetected In Discrete Foci Together With Pml And Numerous Dna Repair Enzymes After Dna Damage By Alkylating AgentsUv Or Gamma IrradiationLocalizes To Sites Of Dna Damage In A H2ax- Independent Manner |
| Alternative Antibody Names | Anti-Dna Topoisomerase 2-Binding Protein 1 antibodyAnti-Dna Topoisomerase Ii-Beta-Binding Protein 1 antibodyAnti-Topbp1 antibodyAnti-Dna Topoisomerase Ii-Binding Protein 1 antibodyAnti-TOPBP1 antibodyAnti-KIAA0259 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
