| Host: |
Rabbit |
| Applications: |
WB/IHC-P/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:100-1:500IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TOPBP1 |
| Gene ID: |
11073 |
| Uniprot ID: |
TOPB1_HUMAN |
| Immunogen Region: |
1253-1522 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2). |
| Immunogen Sequence: |
KIITIEETHEELKKQYIFQL SSLNPQERIDYCHLIEKLGG LVIEKQCFDPTCTHIVVGHP LRNEKYLASVAAGKWVLHRS YLEACRTAGHFVQEEDYEWG SSSILDVLTGINVQQRRLAL AAMRWRKKIQQRQESGIVEG AFSGWKVILHVDQSREAGFK RLLQSGGAKVLPGHSVPLFK EATHLFSDLNKLKPDDSGVN IAEAAAQNVYCLRTEYIADY LMQESPPHVENYCLPEAIS |
| Tissue Specificity | Highly expressed in heart, brain, placenta, lung and kidney. |
| Post Translational Modifications | Phosphorylated on serine and threonine residues in response to X-ray irradiation. Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. Deubiquitinated by USP13.leading to TOPBP1 stabilizion and activation of the ATR-TOPBP1 axis pathway. |
| Function | Scaffold protein that acts as a key protein-protein adapter in DNA replication and DNA repair. Composed of multiple BRCT domains, which specifically recognize and bind phosphorylated proteins, bringing proteins together into functional combinations. Required for DNA replication initiation but not for the formation of pre-replicative complexes or the elongation stages. Necessary for the loading of replication factors onto chromatin, including GMNC, CDC45, DNA polymerases and components of the GINS complex. Plays a central role in DNA repair by bridging proteins and promoting recruitment of proteins to DNA damage sites. Involved in double-strand break (DSB) repair via homologous recombination in S-phase by promoting the exchange between the DNA replication factor A (RPA) complex and RAD51. Mechanistically, TOPBP1 is recruited to DNA damage sites in S-phase via interaction with phosphorylated HTATSF1, and promotes the loading of RAD51, thereby facilitating RAD51 nucleofilaments formation and RPA displacement, followed by homologous recombination. Involved in microhomology-mediated end-joining (MMEJ) DNA repair by promoting recruitment of polymerase theta (POLQ) to DNA damage sites during mitosis. MMEJ is an alternative non-homologous end-joining (NHEJ) machinery that takes place during mitosis to repair DSBs in DNA that originate in S-phase. Recognizes and binds POLQ phosphorylated by PLK1, enabling its recruitment to DSBs for subsequent repair. Involved in G1 DNA damage checkpoint by acting as a molecular adapter that couples TP53BP1 and the 9-1-1 complex. In response to DNA damage, triggers the recruitment of checkpoint signaling proteins on chromatin, which activate the CHEK1 signaling pathway and block S-phase progression. Acts as an activator of the kinase activity of ATR. Also required for chromosomal stability when DSBs occur during mitosis by forming filamentous assemblies that bridge MDC1 and tether broken chromosomes during mitosis. Together with CIP2A, plays an essential role in the response to genome instability generated by the presence of acentric chromosome fragments derived from shattered chromosomes within micronuclei. Micronuclei, which are frequently found in cancer cells, consist of chromatin surrounded by their own nuclear membrane: following breakdown of the micronuclear envelope, a process associated with chromothripsis, the CIP2A-TOPBP1 complex tethers chromosome fragments during mitosis to ensure clustered segregation of the fragments to a single daughter cell nucleus, facilitating re-ligation with limited chromosome scattering and loss. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters, thereby down-regulating E2F1 activity and inhibiting E2F1-dependent apoptosis during G1/S transition and after DNA damage. |
| Protein Name | Dna Topoisomerase 2-Binding Protein 1Dna Topoisomerase Ii-Beta-Binding Protein 1Topbp1Dna Topoisomerase Ii-Binding Protein 1 |
| Database Links | Reactome: R-HSA-5685938Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9709570 |
| Cellular Localisation | NucleusChromosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle PoleLocalizes To Sites Of Dna DamageSuch As Double-Stranded Breaks (Dsbs)Recruited To Dna Double-Strand Break (Dsbs) During S-Phase Following Interaction With Phosphorylated Htatsf1Recruited To Dsbs During Mitosis Following Interaction With Phosphorylated Mdc1Has A Uniform Nuclear Distribution During G PhaseColocalizes With Brca1 At Stalled Replication Forks During S PhaseIn Mitotic Cells It Colocalizes With Brca1 At Spindle Poles And Centrosomes During Metaphase And AnaphaseDetected In Discrete Foci Together With Pml And Numerous Dna Repair Enzymes After Dna Damage By Alkylating AgentsUv Or Gamma IrradiationDetected On Unpaired Autosomes In Meiotic Prophase CellsDetected On X And Y Chromosomes During Later Stages Of ProphaseColocalizes With Atr And H2ax At Unsynapsed Chromosome Cores During ProphaseLocalizes To Broken Chromosomes Within Micronuclei During Interphase And Following ChromothripsisLocalization To Broken Chromosomes Is Mainly Independent Of Mdc1 |
| Alternative Antibody Names | Anti-Dna Topoisomerase 2-Binding Protein 1 antibodyAnti-Dna Topoisomerase Ii-Beta-Binding Protein 1 antibodyAnti-Topbp1 antibodyAnti-Dna Topoisomerase Ii-Binding Protein 1 antibodyAnti-TOPBP1 antibodyAnti-KIAA0259 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
