| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TMEM27 (1-222) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CLTRN |
| Gene ID: |
57393 |
| Uniprot ID: |
CLTRN_HUMAN |
| Immunogen Region: |
1-222 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human TMEM27 (NP_065716.1). |
| Immunogen Sequence: |
MLWLLFFLVTAIHAELCQPG AENAFKVRLSIRTALGDKAY AWDTNEEYLFKAMVAFSMRK VPNREATEISHVLLCNVTQR VSFWFVVTDPSKNHTLPAVE VQSAIRMNKNRINNAFFLND QTLEFLKIPSTLAPPMDPSV PIWIIIFGVIFCIIIVAIAL LILSGIWQRRRKNKEPSEVD DAEDKCENMITIENGIPSDP LDMKGGHINDAFMTEDERLT PL |
| Tissue Specificity | Kidney.collecting ducts. Pancreas.beta cells of islets. |
| Post Translational Modifications | Glycosylated. Glycosylation is required for plasma membrane localization and for its cleavage by BACE2. Proteolytically processed in pancreatic beta cells by BACE2 leading to the generation and extracellular release of soluble CLTRN, and a corresponding cell-associated C-terminal fragment which is later cleaved by gamma-secretase. This shedding process inactivates CLTRN. Three cleavage sites have been identified for BACE2, two clustered sites after Phe-116 and Leu-118 and a more membrane proximal site at Phe-125.the preferred BACE2 cleavage site seems to be between Phe-125 and Leu-126, Phe-116 and Leu-118 act as alternative sites. |
| Function | Plays an important role in amino acid transport by acting as binding partner of amino acid transporters SLC6A18 and SLC6A19, regulating their trafficking on the cell surface and their amino acid transporter activity. May also play a role in trafficking of amino acid transporters SLC3A1 and SLC7A9 to the renal cortical cell membrane. Regulator of SNARE complex function. Stimulator of beta cell replication. |
| Protein Name | CollectrinTransmembrane Protein 27 |
| Database Links | Reactome: R-HSA-264876 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLocalizes To The Brush Border Membranes Of Cells In The Proximal Tubules Of KidneyColocalizes With Slc6a19 In The Early Proximal S1 Tubule |
| Alternative Antibody Names | Anti-Collectrin antibodyAnti-Transmembrane Protein 27 antibodyAnti-CLTRN antibodyAnti-TMEM27 antibodyAnti-UNQ679 antibodyAnti-PRO1312 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
