| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-TIR domain-containing adapter molecule 1 (663-712 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC-P 1:50-200 ELISA 1:10000-20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TICAM1 |
| Gene ID: | 148022 |
| Uniprot ID: | TCAM1_HUMAN |
| Immunogen Region: | 663-712 aa |
| Specificity: | TRIF Polyclonal Antibody detects endogenous levels of TRIF |
| Immunogen: | Synthesized peptide derived from TRIF at the amino acid range 663-712 |
| Post Translational Modifications | Phosphorylated by TBK1. Following activation, phosphorylated by TBK1 at Ser-210 in the pLxIS motif. The phosphorylated pLxIS motif constitutes an IRF3-binding motif, leading to recruitment of the transcription factor IRF3 to induce type-I interferons and other cytokines. Polyubiquitinated at Lys-229 by TRIM38 with 'Lys-48'-linked chains, leading to proteasomal degradation. Polyubiquitinated with 'Lys-6'- and 'Lys-33'-linked chains in a TRIM8-dependent manner.ubiquitination disrupts the interaction with TBK1 and subsequent interferon production. (Microbial infection) Cleaved and degraded by hepatitis A virus (HAV) protein 3CD allowing the virus to disrupt host TLR3 signaling. (Microbial infection) Cleaved by CVB3 protease 3C allowing the virus to disrupt host TLR3 signaling. (Microbial infection) Cleaved by Seneca Valley virus protease 3C allowing the virus to disrupt host TLR3 signaling. (Microbial infection) Cleaved by protease 3C of human enterovirus D68 (EV68) allowing the virus to disrupt host TLR3 signaling. (Microbial infection) Cleaved by HCV protease NS3/4A, thereby disrupting TLR3 signaling and preventing the establishment of an antiviral state. |
| Function | Involved in innate immunity against invading pathogens. Adapter used by TLR3, TLR4 (through TICAM2) and TLR5 to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Phosphorylation by TBK1 on the pLxIS motif leads to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent immunity against invading pathogens. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines. |
| Protein Name | Tir Domain-Containing Adapter Molecule 1 Ticam-1 Proline-Rich - Vinculin And Tir Domain-Containing Protein B Putative Nf-Kappa-B-Activating Protein 502h Toll-Interleukin-1 Receptor Domain-Containing Adapter Protein Inducing Interferon Beta Myd88-3 Tir Domain-Containing Adapter Protein Inducing Ifn-Beta |
| Database Links | Reactome: R-HSA-140534 Reactome: R-HSA-166166 Reactome: R-HSA-168164 Reactome: R-HSA-168927 Reactome: R-HSA-1810476 Reactome: R-HSA-2562578 Reactome: R-HSA-5602566 Reactome: R-HSA-5602571 Reactome: R-HSA-9013957 Reactome: R-HSA-9013973 Reactome: R-HSA-9014325 Reactome: R-HSA-936964 Reactome: R-HSA-937041 Reactome: R-HSA-937072 Reactome: R-HSA-975163 Reactome: R-HSA-9824878 Reactome: R-HSA-9828211 |
| Cellular Localisation | Cytoplasmic Vesicle Autophagosome Cytoplasm Cytosol Mitochondrion Colocalizes With Ubqln1 In The Autophagosome Colocalizes In The Cytosol With Ddx1 Ddx21 And Dhx36 Colocalizes In The Mitochondria With Ddx1 And Poly(I:c) Rna Ligand The Multi-Helicase-Ticam1 Complex May Translocate To The Mitochondria Upon Poly(I:c) Rna Ligand Stimulation |
| Alternative Antibody Names | Anti-Tir Domain-Containing Adapter Molecule 1 antibody Anti-Ticam-1 antibody Anti-Proline-Rich - Vinculin And Tir Domain-Containing Protein B antibody Anti-Putative Nf-Kappa-B-Activating Protein 502h antibody Anti-Toll-Interleukin-1 Receptor Domain-Containing Adapter Protein Inducing Interferon Beta antibody Anti-Myd88-3 antibody Anti-Tir Domain-Containing Adapter Protein Inducing Ifn-Beta antibody Anti-TICAM1 antibody Anti-PRVTIRB antibody Anti-TRIF antibody |
Information sourced from Uniprot.org