• Western blot analysis of extracts of Rat skeletal muscle, using TBP antibody (STJ118864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-TBP antibody (160-319) (STJ118864)

SKU:
STJ118864

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Host: Rabbit
Applications: IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TBP (160-319) is suitable for use in Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TBP
Gene ID: 6908
Uniprot ID: TBP_HUMAN
Immunogen Region: 160-319
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 160-319 of human TBP (NP_001165556.1).
Immunogen Sequence: LKTIALRARNAEYNPKRFAA VIMRIREPRTTALIFSSGKM VCTGAKSEEQSRLAARKYAR VVQKLGFPAKFLDFKIQNMV GSCDVKFPIRLEGLVLTHQQ FSSYEPELFPGLIYRMIKPR IVLLIFVSGKVVLTGAKVRA EIYEAFENIYPILKGFRKTT
Tissue Specificity Widely expressed, with levels highest in the testis and ovary.
Function The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TBP forms the TFIID-A module together with TAF3 and TAF5. TBP is a general transcription factor that functions at the core of the TFIID complex. During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box. Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
Protein Name Tata-Box-Binding Protein
Tata Sequence-Binding Protein
Tata-Binding Factor
Tata-Box Factor
Transcription Initiation Factor Tfiid Tbp Subunit
Database Links Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-427359
Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-674695
Reactome: R-HSA-6804756
Reactome: R-HSA-6807505
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-73863
Reactome: R-HSA-749476
Reactome: R-HSA-75953
Reactome: R-HSA-76042
Reactome: R-HSA-76061
Reactome: R-HSA-76066
Reactome: R-HSA-76071
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Tata-Box-Binding Protein antibody
Anti-Tata Sequence-Binding Protein antibody
Anti-Tata-Binding Factor antibody
Anti-Tata-Box Factor antibody
Anti-Transcription Initiation Factor Tfiid Tbp Subunit antibody
Anti-TBP antibody
Anti-GTF2D1 antibody
Anti-TF2D antibody
Anti-TFIID antibody

Information sourced from Uniprot.org

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