• Western blot analysis of various lysates, using Tafazzin/ TAZ Rabbit polyclonal antibody (STJ114595) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-TAZ antibody (174-248) (STJ114595)

SKU:
STJ114595

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tafazzin/TAZ (174-248) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TAFAZZIN
Gene ID: 6901
Uniprot ID: TAZ_HUMAN
Immunogen Region: 174-248
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 174-248 of human Tafazzin/TAZ (NP_851830.1).
Immunogen Sequence: NDVLPNSPPYFPRFGQKITV LIGKPFSALPVLERLRAENK SAVEMRKALTDFIQEEFQHL KTQAEQLHNHLQPGR
Tissue Specificity High levels in cardiac and skeletal muscle. Up to 10 isoforms can be present in different amounts in different tissues. Most isoforms are ubiquitous. Isoforms that lack the N-terminus are found in leukocytes and fibroblasts, but not in heart and skeletal muscle. Some forms appear restricted to cardiac and skeletal muscle or to leukocytes.
Function Acyltransferase required to remodel newly synthesized phospholipid cardiolipin (1',3'-bis-1,2-diacyl-sn-glycero-3-phospho-glycerol or CL), a key component of the mitochondrial inner membrane, with tissue specific acyl chains necessary for adequate mitochondrial function. Its role in cellular physiology is to improve mitochondrial performance. CL is critical for the coassembly of lipids and proteins in mitochondrial membranes, for instance, remodeling of the acyl groups of CL in the mitochondrial inner membrane affects the assembly and stability of respiratory chain complex IV and its supercomplex forms. Catalyzes the transacylation between phospholipids and lysophospholipids, with the highest rate being between phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) and CL. Catalyzes both 1-acyl-sn-glycero-3-phosphocholine (lysophosphatidylcholine or LPC) reacylation and PC-CL transacylation, that means, it exchanges acyl groups between CL and PC by a combination of forward and reverse transacylations. Also catalyzes transacylations between other phospholipids such as phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) and CL, between PC and PE, and between PC and phosphatidate (1,2-diacyl-sn-glycero-3-phosphate or PA), although at lower rate. Not regiospecific, it transfers acyl groups into any of the sn-1 and sn-2 positions of the monolysocardiolipin (MLCL), which is an important prerequisite for uniformity and symmetry in CL acyl distribution. Cannot transacylate dilysocardiolipin (DLCL), thus, the role of MLCL is limited to that of an acyl acceptor. CoA-independent, it can reshuffle molecular species within a single phospholipid class. Redistributes fatty acids between MLCL, CL, and other lipids, which prolongs the half-life of CL. Its action is completely reversible, which allows for cyclic changes, such as fission and fusion or bending and flattening of the membrane. Hence, by contributing to the flexibility of the lipid composition, it plays an important role in the dynamics of mitochondria membranes. Essential for the final stage of spermatogenesis, spermatid individualization. Required for the initiation of mitophagy. Required to ensure progression of spermatocytes through meiosis. Exon 7 of human tafazzin is essential for catalysis. Isoform 1: Catalyzes the transacylation between lysophosphatidate (such as 1-acyl-sn-glycero-3-phosphate) and phosphatidylglycerol (1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)). Contributes to cardiolipin (1',3'-bis-1,2-diacyl-sn-glycero-3-phospho-glycerol or CL) remodeling. Isoform 3: Catalyzes the transacylation between lysophospholipids and phospholipids, and plays a fundamental role in cardiolipin (1',3'-bis-1,2-diacyl-sn-glycero-3-phospho-glycerol or CL) metabolism and remodeling. Isoform 5: Catalytically inactive. Isoform 7: Catalytically inactive.
Protein Name Tafazzin
Taz
Protein G4.5
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-1482798
Cellular Localisation Mitochondrion Outer Membrane
Peripheral Membrane Protein
Intermembrane Side
Mitochondrion Inner Membrane
Isoform 1: Mitochondrion Membrane
Isoform 2: Cytoplasm
Isoform 3: Mitochondrion Membrane
Isoform 5: Mitochondrion Membrane
Isoform 6: Cytoplasm
Isoform 7: Mitochondrion Membrane
Isoform 8: Cytoplasm
Isoform 9: Cytoplasm
Alternative Antibody Names Anti-Tafazzin antibody
Anti-Taz antibody
Anti-Protein G4.5 antibody
Anti-TAFAZZIN antibody
Anti-EFE2 antibody
Anti-G4.5 antibody
Anti-TAZ antibody

Information sourced from Uniprot.org

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