• Western blot analysis of extracts of various cell lines, using Somatostatin (SST) antibody (STJ111633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of paraffin-embedded mouse pancreas using Somatostatin (SST) Rabbit polyclonal antibody (STJ111633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat brain, using Somatostatin (SST) antibody (STJ111633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of paraffin-embedded rat pancreas using Somatostatin (SST) Rabbit polyclonal antibody (STJ111633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human appendix using Somatostatin (SST) antibody (STJ111633) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Somatostatin (SST) in paraffin-embedded mouse pancreas using Somatostatin (SST) Rabbit polyclonal antibody (STJ111633) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse pancreas using Somatostatin (SST) antibody (STJ111633) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Somatostatin (SST) in paraffin-embedded human appendix using Somatostatin (SST) Rabbit polyclonal antibody (STJ111633) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat pancreas using Somatostatin (SST) rabbit polyclonal antibody (STJ111633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat brain, using Somatostatin (SST) Rabbit polyclonal antibody (STJ111633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 600ug extracts of mouse brain cells using 3ug Somatostatin (SST) antibody (STJ111633). Western blot was performed from the immunoprecipitate using SST antibody (STJ111633) at a dilition of 1:1000.

Anti-SST antibody (23-116) (STJ111633)

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STJ111633

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SST (23-116) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SST
Gene ID: 6750
Uniprot ID: SMS_HUMAN
Immunogen Region: 23-116
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-116 of human SST (NP_001039.1).
Immunogen Sequence: TGAPSDPRLRQFLQKSLAAA AGKQELAKYFLAELLSEPNQ TENDALEPEDLSQAAEQDEM RLELQRSANSNPAMAPRERK AGCKNFFWKTFTSC
Post Translational Modifications C-terminal amidation of the neuronostatin peptide is required for its biological activity, including for the regulation of mean arterial pressure.
Function Somatostatin-14: Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH.the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin. Neuronostatin: May enhance low-glucose-induced glucagon release by pancreatic alpha cells. This effect may be mediated by binding to GPR107 and PKA activation. May regulate cardiac contractile function. May compromise cardiomyocyte viability. In the central nervous system, may impair memory retention and may affect hippocampal excitability. May also have anxiolytic and anorexigenic effects. May play a role in arterial pressure regulation. May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH.
Protein Name Somatostatin
Growth Hormone Release-Inhibiting Factor Cleaved Into - Somatostatin-28 - Somatostatin-14
Sst-14 - Neuronostatin
Nst
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-9022702
Cellular Localisation Secreted
Alternative Antibody Names Anti-Somatostatin antibody
Anti-Growth Hormone Release-Inhibiting Factor Cleaved Into - Somatostatin-28 - Somatostatin-14 antibody
Anti-Sst-14 - Neuronostatin antibody
Anti-Nst antibody
Anti-SST antibody

Information sourced from Uniprot.org

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