| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPHK1 |
| Gene ID: | 8877 |
| Uniprot ID: | SPHK1_HUMAN |
| Immunogen Region: | 50-384 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-384 of human SPHK1 (NP_001136074.1). |
| Immunogen Sequence: | TLMLTERRNHARELVRSEEL GRWDALVVMSGDGLMHEVVN GLMERPDWETAIQKPLCSLP AGSGNALAASLNHYAGYEQV TNEDLLTNCTLLLCRRLLSP MNLLSLHTASGLRLFSVLSL AWGFIADVDLESEKYRRLGE MRFTLGTFLRLAALRTYRGR LAYLPVGRVGSKTPASPVVV QQGPVDAHLVPLEEPVPSHW TVVPDEDFVLVLALLHSHLG SEMFAAPMGRCAAGVMHLF |
| Tissue Specificity | Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level). |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA. Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. |
| Protein Name | Sphingosine Kinase 1 Sk 1 Spk 1 Acetyltransferase Sphk1 |
| Database Links | Reactome: R-HSA-1660661 Reactome: R-HSA-390471 Reactome: R-HSA-5218921 Reactome: R-HSA-9009391 Reactome: R-HSA-9833482 |
| Cellular Localisation | Cytoplasm Nucleus Cell Membrane Endosome Membrane Peripheral Membrane Protein Membrane Clathrin-Coated Pit Synapse Translocated From The Cytoplasm To The Plasma Membrane In A Cib1-Dependent Manner Binds To Membranes Containing Negatively Charged Lipids But Not Neutral Lipids Recruited To Endocytic Membranes By Sphingosine Where Promotes Membrane Fusion |
| Alternative Antibody Names | Anti-Sphingosine Kinase 1 antibody Anti-Sk 1 antibody Anti-Spk 1 antibody Anti-Acetyltransferase Sphk1 antibody Anti-SPHK1 antibody Anti-SK1 antibody Anti-SPHK antibody Anti-SPK antibody |
Information sourced from Uniprot.org