| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SNX5 |
| Gene ID: | 27131 |
| Uniprot ID: | SNX5_HUMAN |
| Immunogen Region: | 145-404 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 145-404 of human SNX5 (NP_055241.1). |
| Immunogen Sequence: | VFLQRLSSHPVLSKDRNFHV FLEYDQDLSVRRKNTKEMFG GFFKSVVKSADEVLFTGVKE VDDFFEQEKNFLINYYNRIK DSCVKADKMTRSHKNVADDY IHTAACLHSLALEEPTVIKK YLLKVAELFEKLRKVEGRVS SDEDLKLTELLRYYMLNIEA AKDLLYRRTKALIDYENSNK ALDKARLKSKDVKLAEAHQQ ECCQKFEQLSESAKEELINF KRKRVAAFRKNLIEMSELE |
| Function | Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Does not have in vitro vesicle-to-membrane remodeling activity. Involved in retrograde transport of lysosomal enzyme receptor IGF2R. May function as link between endosomal transport vesicles and dynactin (Probable). Plays a role in the internalization of EGFR after EGF stimulation (Probable). Involved in EGFR endosomal sorting and degradation.the function involves PIP5K1C isoform 3 and is retromer-independent. Together with PIP5K1C isoform 3 facilitates HGS interaction with ubiquitinated EGFR, which initiates EGFR sorting to intraluminal vesicles (ILVs) of the multivesicular body for subsequent lysosomal degradation (Probable). Involved in E-cadherin sorting and degradation.inhibits PIP5K1C isoform 3-mediated E-cadherin degradation. Plays a role in macropinocytosis. |
| Protein Name | Sorting Nexin-5 |
| Database Links | Reactome: R-HSA-432722 |
| Cellular Localisation | Endosome Early Endosome Early Endosome Membrane Peripheral Membrane Protein Cytoplasmic Side Cell Membrane Cytoplasmic Vesicle Membrane Cytoplasm Cell Projection Phagocytic Cup Ruffle Recruited To The Plasma Membrane After Egf Stimulation Which Leads To Increased Levels Of Phosphatidylinositol 3 4-Bisphosphate (Pdtins(3 4)P2) Detected On Macropinosomes Targeted To Membrane Ruffles In Response To Egfr Stimulation |
| Alternative Antibody Names | Anti-Sorting Nexin-5 antibody Anti-SNX5 antibody |
Information sourced from Uniprot.org