• Immunofluorescence analysis of HeLa cells using Smad5 Polyclonal Antibody (STJ115958) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using SMAD5 antibody (STJ115958) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using SMAD5 antibody (STJ115958) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Smad5 antibody (STJ115958) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Smad5 antibody (200-300) (STJ115958)

SKU:
STJ115958

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Smad5 (200-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMAD5
Gene ID: 4090
Uniprot ID: SMAD5_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Smad5 (NP_005894.3).
Immunogen Sequence: STYPNSPASSGPGSPFQLPA DTPPPAYMPPDDQMGQDNSQ PMDTSNNMIPQIMPSISSRD VQPVAYEEPKHWCSIVYYEL NNRVGEAFHASSTSVLVDGF T
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Function Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis.
Protein Name Mothers Against Decapentaplegic Homolog 5
Mad Homolog 5
Mothers Against Dpp Homolog 5
Jv5-1
Smad Family Member 5
Smad 5
Smad5
Hsmad5
Database Links Reactome: R-HSA-201451
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Cytoplasmic In The Absence Of Ligand
Migrates To The Nucleus When Complexed With Smad4
Alternative Antibody Names Anti-Mothers Against Decapentaplegic Homolog 5 antibody
Anti-Mad Homolog 5 antibody
Anti-Mothers Against Dpp Homolog 5 antibody
Anti-Jv5-1 antibody
Anti-Smad Family Member 5 antibody
Anti-Smad 5 antibody
Anti-Smad5 antibody
Anti-Hsmad5 antibody
Anti-SMAD5 antibody
Anti-MADH5 antibody

Information sourced from Uniprot.org

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