| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-SLC46A1/PCFT (233-247) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000. WB: Approx 50kDa band observed in Human Kidney lysates (calculated MW of 49.8kDa according to NP_542400.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room tempera |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SLC46A1 |
| Gene ID: | 113235 |
| Uniprot ID: | PCFT_HUMAN |
| Immunogen Region: | 233-247 |
| Accession Number: | NP_542400.2 |
| Immunogen Sequence: | LKEPKSTRLFTFRH |
| Function | Proton-coupled folate symporter that mediates folate absorption using an H(+) gradient as a driving force. Involved in the intestinal absorption of folates at the brush-border membrane of the proximal jejunum, and the transport from blood to cerebrospinal fluid across the choroid plexus. Functions at acidic pH via alternate outward- and inward-open conformation states. Protonation of residues in the outward open state primes the protein for transport. Binding of folate promotes breaking of salt bridge network and subsequent closure of the extracellular gate, leading to the inward-open state and release of protons and folate. Also able to transport antifolate drugs, such as methotrexate and pemetrexed, which are established treatments for cancer and autoimmune diseases. Involved in FOLR1-mediated endocytosis by serving as a route of export of folates from acidified endosomes. Also acts as a lower-affinity, pH-independent heme carrier protein and constitutes the main importer of heme in the intestine. Imports heme in the retina and retinal pigment epithelium, in neurons of the hippocampus, in hepatocytes and in the renal epithelial cells. Hence, participates in the trafficking of heme and increases intracellular iron content. Isoform 2: Inactive isoform which is not able to mediate proton-coupled folate transport. |
| Protein Name | Proton-Coupled Folate Transporter Hspcft Hpcft Heme Carrier Protein 1 Pcft/Hcp1 Solute Carrier Family 46 Member 1 |
| Database Links | Reactome: R-HSA-196757 Reactome: R-HSA-917937 Reactome: R-HSA-9707616 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Apical Cell Membrane Basolateral Cell Membrane Endosome Membrane Cytoplasm Localizes To The Apical Membrane Of Intestinal Cells In Iron-Deficient Cells While It Resides In The Cytoplasm In Iron-Replete Cells Localizes To The Basolateral Membrane Of Choroid Plexus |
| Alternative Antibody Names | Anti-Proton-Coupled Folate Transporter antibody Anti-Hspcft antibody Anti-Hpcft antibody Anti-Heme Carrier Protein 1 antibody Anti-Pcft/Hcp1 antibody Anti-Solute Carrier Family 46 Member 1 antibody Anti-SLC46A1 antibody Anti-G21 antibody Anti-HCP1 antibody Anti-PCFT antibody |
Information sourced from Uniprot.org