Western blot analysis of extracts of various cell lines, using SLC25A13 antibody (STJ114431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SLC25A13 antibody (1-300) (STJ114431)

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STJ114431
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC25A13
Gene ID: 10165
Uniprot ID: S2513_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SLC25A13 (NP_001153682.1).
Immunogen Sequence: MAAAKVALTKRADPAELRTI FLKYASIEKNGEFFMSPNDF VTRYLNIFGESQPNPKTVEL LSGVVDQTKDGLISFQEFVA FESVLCAPDALFMVAFQLFD KAGKGEVTFEDVKQVFGQTT IHQHIPFNWDSEFVQLHFGK ERKRHLTYAEFTQFLLEIQL EHAKQAFVQRDNARTGRVTA IDFRDIMVTIRPHVLTPFVE ECLVAAAGGTTSHQVSFSYF NGFNSLLNNMELIRKIYST
Tissue Specificity High levels in liver and low levels in kidney, pancreas, placenta, heart and brain.
Function Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle. Also mediates the uptake of L-cysteinesulfinate by mitochondria in exchange of L-glutamate and proton. Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation.
Protein Name Electrogenic Aspartate/Glutamate Antiporter Slc25a13 - Mitochondrial
Calcium-Binding Mitochondrial Carrier Protein Aralar2
Aralar-Related Gene 2
Aralar2
Citrin
Mitochondrial Aspartate Glutamate Carrier 2
Solute Carrier Family 25 Member 13
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-8963693
Reactome: R-HSA-9856872
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Electrogenic Aspartate/Glutamate Antiporter Slc25a13 - Mitochondrial antibody
Anti-Calcium-Binding Mitochondrial Carrier Protein Aralar2 antibody
Anti-Aralar-Related Gene 2 antibody
Anti-Aralar2 antibody
Anti-Citrin antibody
Anti-Mitochondrial Aspartate Glutamate Carrier 2 antibody
Anti-Solute Carrier Family 25 Member 13 antibody
Anti-SLC25A13 antibody

Information sourced from Uniprot.org

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