• Western blot analysis of extracts of various cell lines, using SLC22A1 antibody (STJ111110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SLC22A1 antibody (43-149) (STJ111110)

SKU:
STJ111110

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC22A1 (43-149) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC22A1
Gene ID: 6580
Uniprot ID: S22A1_HUMAN
Immunogen Region: 43-149
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 43-149 of human SLC22A1 (O15245).
Immunogen Sequence: GFTPDHHCQSPGVAELSQRC GWSPAEELNYTVPGLGPAGE AFLGQCRRYEVDWNQSALSC VDPLASLATNRSHLPLGPCQ DGWVYDTPGSSIVTEFNLVC ADSWKLD
Tissue Specificity Widely expressed with high level in liver. In liver, expressed around the central vein. Expressed in kidney. Expressed in small intestine enterocytes. Localized to peritubular myoid cells, Leydig cells and moderately to the basal membrane of Sertoli cells in testes. Expressed in tracheal and bronchial ciliated epithelium in the respiratory tract. Also expressed in skeletal muscle, stomach, spleen, heart, placentacolon, brain, granulycytes and lympohocytes. Isoform 1: Expressed in liver and in glial cell lines. Isoform 2: Expressed in liver and in glial cell lines. Isoform 3: Expressed in glial cell lines. Not expressed in liver. Isoform 4: Expressed in glial cell lines. Not expressed in liver.
Post Translational Modifications Phosphorylated.
Function Electrogenic voltage-dependent transporter that mediates the transport of a variety of organic cations such as endogenous bioactive amines, cationic drugs and xenobiotics. Functions as a pH- and Na(+)-independent, bidirectional transporter. Cation cellular uptake or release is driven by the electrochemical potential (i.e. membrane potential and concentration gradient) and substrate selectivity. Hydrophobicity is a major requirement for recognition in polyvalent substrates and inhibitors. Primarily expressed at the basolateral membrane of hepatocytes and proximal tubules and involved in the uptake and disposition of cationic compounds by hepatic and renal clearance from the blood flow. Most likely functions as an uptake carrier in enterocytes contributing to the intestinal elimination of organic cations from the systemic circulation. Transports endogenous monoamines such as N-1-methylnicotinamide (NMN), guanidine, histamine, neurotransmitters dopamine, serotonin and adrenaline. Also transports natural polyamines such as spermidine, agmatine and putrescine at low affinity, but relatively high turnover. Involved in the hepatic uptake of vitamin B1/thiamine, hence regulating hepatic lipid and energy metabolism. Mediates the bidirectional transport of acetylcholine (ACh) at the apical membrane of ciliated cell in airway epithelium, thereby playing a role in luminal release of ACh from bronchial epithelium. Transports dopaminergic neuromodulators cyclo(his-pro) and salsolinol with lower efficency. Also capable of transporting non-amine endogenous compounds such as prostaglandin E2 (PGE2) and prostaglandin F2-alpha (PGF2-alpha). May contribute to the transport of cationic compounds in testes across the blood-testis-barrier (Probable). Also involved in the uptake of xenobiotics tributylmethylammonium (TBuMA), quinidine, N-methyl-quinine (NMQ), N-methyl-quinidine (NMQD) N-(4,4-azo-n-pentyl)-quinuclidine (APQ), azidoprocainamide methoiodide (AMP), N-(4,4-azo-n-pentyl)-21-deoxyajmalinium (APDA) and 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP). Isoform 1: Mediates the uptake of 1-methyl-4-phenylpyridinium (MPP(+)). Isoform 2: Not able to uptake 1-methyl-4-phenylpyridinium (MPP(+)). Isoform 3: Not able to uptake 1-methyl-4-phenylpyridinium (MPP(+)). Isoform 4: Not able to uptake 1-methyl-4-phenylpyridinium (MPP(+)).
Protein Name Solute Carrier Family 22 Member 1
Organic Cation Transporter 1
Hoct1
Database Links Reactome: R-HSA-112311
Reactome: R-HSA-181430
Reactome: R-HSA-2161517
Reactome: R-HSA-442660
Reactome: R-HSA-549127
Reactome: R-HSA-9793528
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Lateral Cell Membrane
Basal Cell Membrane
Cell Membrane
Localized To The Sinusoidal/Basolateral Membrane Of Hepatocytes
Mainly Localized To The Basolateral Membrane Of Renal Proximal Tubular Cells
However
Also Identified At The Apical Side Of Proximal Tubular Cells
Mainly Expressed At The Lateral Membrane Of Enterocytes
Also Observed At The Apical Side Of Enterocytes
Localized To The Luminal/Apical Membrane Of Ciliated Epithelial Cells In Bronchi
Localized To The Basal Membrane Of Sertoli Cells
Alternative Antibody Names Anti-Solute Carrier Family 22 Member 1 antibody
Anti-Organic Cation Transporter 1 antibody
Anti-Hoct1 antibody
Anti-SLC22A1 antibody
Anti-OCT1 antibody

Information sourced from Uniprot.org

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