| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SLC17A6 |
| Gene ID: | 57084 |
| Uniprot ID: | VGLU2_HUMAN |
| Immunogen Region: | 500-582 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 500-582 of human SLC17A6 (NP_065079.1). |
| Immunogen Sequence: | GEKQPWADPEETSEEKCGFI HEDELDEETGDITQNYINYG TTKSYGATTQANGGWPSGWE KKEEFVQGEVQDSHSYKDRV DYS |
| Tissue Specificity | Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus. |
| Function | Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate. At the synaptic vesicle membrane, mainly functions as a uniporter which transports preferentially L-glutamate but also, phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane. In addition, functions as a chloride channel that allows the chloride permeation through the synaptic vesicle membrane therefore affects the proton electrochemical gradient and promotes synaptic vesicles acidification. Moreover, functions as a vesicular K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake. The vesicular H(+)/H(+) antiport activity is electroneutral. At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (Probable). The symporter activity is driven by an inside negative membrane potential and is electrogenic (Probable). Also involved in the regulation of retinal hyaloid vessel regression during postnatal development. May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas. |
| Protein Name | Vesicular Glutamate Transporter 2 Vglut2 Differentiation-Associated Bnpi Differentiation-Associated Na(+-Dependent Inorganic Phosphate Cotransporter Solute Carrier Family 17 Member 6 |
| Database Links | Reactome: R-HSA-428643 |
| Cellular Localisation | Cytoplasmic Vesicle Secretory Vesicle Synaptic Vesicle Membrane Multi-Pass Membrane Protein Synapse Synaptosome Cell Membrane |
| Alternative Antibody Names | Anti-Vesicular Glutamate Transporter 2 antibody Anti-Vglut2 antibody Anti-Differentiation-Associated Bnpi antibody Anti-Differentiation-Associated Na(+-Dependent Inorganic Phosphate Cotransporter antibody Anti-Solute Carrier Family 17 Member 6 antibody Anti-SLC17A6 antibody Anti-DNPI antibody Anti-VGLUT2 antibody |
Information sourced from Uniprot.org