| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SLC12A5 (945-1025) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLC12A5 |
| Gene ID: |
57468 |
| Uniprot ID: |
S12A5_HUMAN |
| Immunogen Region: |
945-1025 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 945-1025 of human SLC12A5 (NP_001128243.1). |
| Immunogen Sequence: |
TKNEREREIQSITDESRGSI RRKNPANTRLRLNVPEETAG DSEEKPEEEVQLIHDQSAPS CPSSSPSPGEEPEGEGETDP E |
| Tissue Specificity | Brain specific. Detected in neuronal cells. |
| Post Translational Modifications | Phosphorylated at Thr-929 and Thr-1030 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity. |
| Function | Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation. |
| Protein Name | Solute Carrier Family 12 Member 5Electroneutral Potassium-Chloride Cotransporter 2K-Cl Cotransporter 2Hkcc2Neuronal K-Cl Cotransporter |
| Database Links | Reactome: R-HSA-426117 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionDendriteDetected On DendritesBut Not On Axons Of Spinal Cord Neurons And At Gphn-Positive Inhibitory Synapses |
| Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 5 antibodyAnti-Electroneutral Potassium-Chloride Cotransporter 2 antibodyAnti-K-Cl Cotransporter 2 antibodyAnti-Hkcc2 antibodyAnti-Neuronal K-Cl Cotransporter antibodyAnti-SLC12A5 antibodyAnti-KCC2 antibodyAnti-KIAA1176 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
