• Western blot analysis of extracts of various cell lines, using SCNN1B antibody (STJ114572) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SCNN1B antibody (406-640) (STJ114572)

SKU:
STJ114572

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCNN1B (406-640) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCNN1B
Gene ID: 6338
Uniprot ID: SCNNB_HUMAN
Immunogen Region: 406-640
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 406-640 of human SCNN1B (NP_000327.2).
Immunogen Sequence: YPLPRGEKYCNNRDFPDWAH CYSDLQMSVAQRETCIGMCK ESCNDTQYKMTISMADWPSE ASEDWIFHVLSQERDQSTNI TLSRKGIVKLNIYFQEFNYR TIEESAANNIVWLLSNLGGQ FGFWMGGSVLCLIEFGEIII DFVWITIIKLVALAKSLRQR RAQASYAGPPPTVAELVEAH TNFGFQPDTAPRSPNTGPYP SEQALPIPGTPPPNYDSLRL QPLDVIESDSEGDAI
Tissue Specificity Detected in placenta, lung and kidney. Expressed in kidney (at protein level).
Post Translational Modifications Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. N-glycosylated. N-glycosylation is required for interaction with BPIFA1.
Function Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Protein Name Amiloride-Sensitive Sodium Channel Subunit Beta
Beta-Nach
Epithelial Na(+ Channel Subunit Beta
Beta-Enac
Enacb
Nonvoltage-Gated Sodium Channel 1 Subunit Beta
Scneb
Database Links Reactome: R-HSA-2672351
Reactome: R-HSA-9730628
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle Membrane
Apical Membrane Of Epithelial Cells
Alternative Antibody Names Anti-Amiloride-Sensitive Sodium Channel Subunit Beta antibody
Anti-Beta-Nach antibody
Anti-Epithelial Na(+ Channel Subunit Beta antibody
Anti-Beta-Enac antibody
Anti-Enacb antibody
Anti-Nonvoltage-Gated Sodium Channel 1 Subunit Beta antibody
Anti-Scneb antibody
Anti-SCNN1B antibody

Information sourced from Uniprot.org

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