HEK293 overexpressing SKAP2 (RC206119) and probed with STJ70135 (mock transfection in first lane) , tested by Origene.

Anti-SCAP2/PRAP antibody (C-Term) (STJ70135)

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STJ70135-100
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-SCAP2/PRAP (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:3000.
WB: Approx. 26+37kDa bands observed in lysates of cell line K562, and approx. 28+38kDa in preliminary testing of U937 cell lysate (calculated MW of 21.6kDa according to NP_001290397.1 and 4
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SKAP2
Gene ID: 8935
Uniprot ID: SKAP2_HUMAN
Immunogen Region: C-Term
Accession Number: NP_003921.2
Immunogen Sequence: GLVPKAYIMEMYDI
Post Translational Modifications Phosphorylated in resting platelets. Phosphorylated by FYN on Tyr-261 upon T-cell activation (Probable). Dephosphorylated on Tyr-75 by PTPN22.
Function May be involved in B-cell and macrophage adhesion processes. In B-cells, may act by coupling the B-cell receptor (BCR) to integrin activation. May play a role in src signaling pathway.
Protein Name Src Kinase-Associated Phosphoprotein 2
Pyk2/Raftk-Associated Protein
Retinoic Acid-Induced Protein 70
Skap55 Homolog
Skap-55hom
Skap-Hom
Src Family-Associated Phosphoprotein 2
Src Kinase-Associated Phosphoprotein 55-Related Protein
Src-Associated Adapter Protein With Ph And Sh3 Domains
Database Links Reactome: R-HSA-391160
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Src Kinase-Associated Phosphoprotein 2 antibody
Anti-Pyk2/Raftk-Associated Protein antibody
Anti-Retinoic Acid-Induced Protein 70 antibody
Anti-Skap55 Homolog antibody
Anti-Skap-55hom antibody
Anti-Skap-Hom antibody
Anti-Src Family-Associated Phosphoprotein 2 antibody
Anti-Src Kinase-Associated Phosphoprotein 55-Related Protein antibody
Anti-Src-Associated Adapter Protein With Ph And Sh3 Domains antibody
Anti-SKAP2 antibody
Anti-PRAP antibody
Anti-RA70 antibody
Anti-SAPS antibody
Anti-SCAP2 antibody
Anti-SKAP55R antibody

Information sourced from Uniprot.org

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