• Western blot analysis of SARS-COV-2 N Protein using SARS-COV-2 N Protein Rabbit pAb (STJ11101210) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.

Anti-SARS-COV-2 N Protein antibody (STJ11101210)

Current Stock:
Host: Rabbit
Applications: WB, ELISA, FC, IHC, IF, IP
Reactivity: SARS-CoV-2
Short Description: Rabbit monoclonal primary antibody against 2019-nCoV.
Clonality: Monoclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at 4°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:1000-1:5000
ELISA: 1:1000-1:5000
FCM: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:200
Immunogen: Recombinant fusion protein of SARS-COV-2 Nucleoprotein.
1 Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
6 months standard