Anti-S100-A9 protein antibody [6G10] (STJA0002914)

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STJA0002914

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Host: Mouse
Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-S100-A9 protein is suitable for use in ELISA research applications.
Clonality: Monoclonal
Clone ID: 6G10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS with 50% glycerol
Purification: NA Protein G purification NA
Dilution Range: ELISA 1-0.2 µg/ml
Storage Instruction: Store at-20 to-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
Gene Symbol: S100A9
Gene ID: 6280
Uniprot ID: S10A9_HUMAN
Specificity: Human S100-A9
Immunogen: Human S100-A9
Post Translational Modifications Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization. S-nitrosylation of Cys-3 is implicated in LDL(ox)-induced S-nitrosylation of GAPDH at 'Cys-247' through a transnitrosylase mechanism involving a iNOS-S100A8/9 complex. Methylation at His-105 by METTL9 reduces zinc-binding without affecting heterodimerization with S100A8.
Function S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect.regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Has transnitrosylase activity.in oxidatively-modified low-densitity lipoprotein (LDL(ox))-induced S-nitrosylation of GAPDH on 'Cys-247' proposed to transfer the NO moiety from NOS2/iNOS to GAPDH via its own S-nitrosylated Cys-3. The iNOS-S100A8/A9 transnitrosylase complex is proposed to also direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a IL-x-C-x-x-DE motif.
Protein Name Protein S100-A9
Calgranulin-B
Calprotectin L1h Subunit
Leukocyte L1 Complex Heavy Chain
Migration Inhibitory Factor-Related Protein 14
Mrp-14
P14
S100 Calcium-Binding Protein A9
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-166058
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5668599
Reactome: R-HSA-5686938
Reactome: R-HSA-6798695
Reactome: R-HSA-6799990
Cellular Localisation Secreted
Cytoplasm
Cytoskeleton
Cell Membrane
Peripheral Membrane Protein
Predominantly Localized In The Cytoplasm
Upon Elevation Of The Intracellular Calcium Level
Translocated From The Cytoplasm To The Cytoskeleton And The Cell Membrane
Upon Neutrophil Activation Or Endothelial Adhesion Of Monocytes
Is Secreted Via A Microtubule-Mediated
Alternative Pathway
Alternative Antibody Names Anti-Protein S100-A9 antibody
Anti-Calgranulin-B antibody
Anti-Calprotectin L1h Subunit antibody
Anti-Leukocyte L1 Complex Heavy Chain antibody
Anti-Migration Inhibitory Factor-Related Protein 14 antibody
Anti-Mrp-14 antibody
Anti-P14 antibody
Anti-S100 Calcium-Binding Protein A9 antibody
Anti-S100A9 antibody
Anti-CAGB antibody
Anti-CFAG antibody
Anti-MRP14 antibody

Information sourced from Uniprot.org

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